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Dual fluorophore expression in mammalian systems
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6
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138
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September 11, 2025
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How can I run channel-wise acquisition programs on an AxioScan 7?
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2
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86
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July 19, 2025
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OlympusFV1000 Operational Help - Error Message - Confocal Microscopy
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4
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187
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May 14, 2025
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How am I obtaining higher energy photons from a fluorescein solution than the ones I'm exciting it with?
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3
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131
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February 28, 2025
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Which eGFP/FITC filter set?
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1
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77
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February 24, 2025
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Guide to Microscopy about Cellular Ploidy Variation
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2
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350
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February 13, 2025
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Andor Zyla for FISH (in situ hibridization)
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0
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65
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January 7, 2025
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Correct orientation of filters - arrow direction?
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4
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265
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December 20, 2024
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Increasing brightness in eGFP channel with wide-pass filter set?
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6
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128
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December 18, 2024
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FRAP acquisition issues - ROI not bleaching fully
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5
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555
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June 8, 2024
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Does 2x scan speed + 2x averaging reduce photobleaching compared to 1x scan speed + 1x averaging (no averaging)?
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4
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426
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February 7, 2024
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Imaging live culture neurons on a Plastic Plate
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2
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564
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December 13, 2023
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Guide to Microscopy about Cellular Ploidy Variation 2
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0
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234
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October 30, 2023
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Membrane dye for fixed samples
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2
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574
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December 18, 2022
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Blurring of light sheet
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3
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503
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September 13, 2022
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New monochrome camera recommendation
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0
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466
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August 12, 2022
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Bacterial staining on formaline fixed spheroids
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0
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403
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January 24, 2022
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ER labeling for live cell imaging
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2
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636
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January 14, 2022
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Cell culture and live cell imaging
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15
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6491
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June 18, 2021
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Recommendations for fluorescent nano-beads for PSF confocal
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7
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1919
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February 18, 2021
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Unspecific signals in fluorescence staining of neutrophil extracellular traps
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2
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782
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February 12, 2021
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mEos versions and other recommendations for point localization in tissue
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0
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502
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July 24, 2020
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Working with genetically encoded Redox sensors?
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0
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492
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July 2, 2020
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Extinction coefficient & quantum yield units?
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4
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1316
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May 26, 2020
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Slicing tissue blocks
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2
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919
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February 14, 2020
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mTurquoise2, mNeonGreen and mCherry in Yeast
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5
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1231
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January 7, 2020
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Possible photoconversion of mCherry?
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7
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1594
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October 25, 2019
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Plant FP - please offer advice on what FP to choose
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4
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753
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October 1, 2019
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Blue fluorescent protein for OMX
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7
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2004
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September 13, 2019
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Does low fluorophore pKa = low pH sensitivity?
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2
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2224
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August 18, 2019
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