ER labeling for live cell imaging

Hi everyone,

I am asking for recommendations on how to fluorescently label the endoplasmic reticulum in live HEK293 cells. Do you have recommendations for any ER trackers? Another protein is tagged with mCherry. So what are some good ER trackers that separate from mCherry? This is for live-cell imaging.

Thank you!

I’d recommend looking trough Addgene or the literature for people that image the ER in live cells, Gia Voeltz is a leader in this area, Jennifer Lippincott-Schwartz used to do a lot off this, and I’m sure there are others. Also look at Thermo (Molecular Probes), they have many organelle markers nowadays. Good luck.

Thank you, James! And the system tells me I must reply at least 20 characters? I just want to say thank you!