Good morning
My name is Matheus, I’m starting out in the area of image analysis and I would like to develop an analysis and identification of polyploid cells in histological slides stained in HE and also with fluorescence.
I would like to know if there was any guide that highlights how to identify and characterize polyploid and multinucleated cells in slides observed using conventional or fluorescence microscopy?
Thanks a lot for the help.
I see it’s been a while since this topic was discussed, but I just wanted to ask—has anyone tried using image analysis software to quantify ploidy variation more automatically? I know some tools can analyze nuclear size and intensity, but I wonder how reliable they are compared to traditional microscopy assessments. Would love to hear if anyone has experience with this or recommendations for good software!
Variations in cellular ploidy can be tricky to analyze, especially when dealing with mixed populations. I’ve found that good image processing tools make a huge difference in quantifying fluorescence intensity accurately. Checking out merkel.co.il might be useful since they have some solid solutions that could help refine your imaging workflow. Proper segmentation and background correction are key when trying to get reliable measurements from fluorescence data.