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How am I obtaining higher energy photons from a fluorescein solution than the ones I'm exciting it with?
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3
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75
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February 28, 2025
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Which eGFP/FITC filter set?
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1
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36
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February 24, 2025
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Guide to Microscopy about Cellular Ploidy Variation
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2
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308
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February 13, 2025
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Andor Zyla for FISH (in situ hibridization)
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0
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39
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January 7, 2025
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Correct orientation of filters - arrow direction?
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4
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84
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December 20, 2024
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Increasing brightness in eGFP channel with wide-pass filter set?
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6
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48
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December 18, 2024
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FRAP acquisition issues - ROI not bleaching fully
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5
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373
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June 8, 2024
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Does 2x scan speed + 2x averaging reduce photobleaching compared to 1x scan speed + 1x averaging (no averaging)?
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4
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355
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February 7, 2024
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Imaging live culture neurons on a Plastic Plate
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2
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457
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December 13, 2023
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Guide to Microscopy about Cellular Ploidy Variation 2
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0
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232
|
October 30, 2023
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Membrane dye for fixed samples
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2
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501
|
December 18, 2022
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Blurring of light sheet
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3
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444
|
September 13, 2022
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New monochrome camera recommendation
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0
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445
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August 12, 2022
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Bacterial staining on formaline fixed spheroids
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0
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372
|
January 24, 2022
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ER labeling for live cell imaging
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2
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592
|
January 14, 2022
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Cell culture and live cell imaging
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15
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5857
|
June 18, 2021
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Recommendations for fluorescent nano-beads for PSF confocal
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7
|
1798
|
February 18, 2021
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Unspecific signals in fluorescence staining of neutrophil extracellular traps
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2
|
729
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February 12, 2021
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mEos versions and other recommendations for point localization in tissue
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0
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492
|
July 24, 2020
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Working with genetically encoded Redox sensors?
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0
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479
|
July 2, 2020
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Extinction coefficient & quantum yield units?
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4
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1223
|
May 26, 2020
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Slicing tissue blocks
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2
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870
|
February 14, 2020
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mTurquoise2, mNeonGreen and mCherry in Yeast
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5
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1139
|
January 7, 2020
|
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Possible photoconversion of mCherry?
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7
|
1460
|
October 25, 2019
|
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Plant FP - please offer advice on what FP to choose
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4
|
702
|
October 1, 2019
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Blue fluorescent protein for OMX
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7
|
1919
|
September 13, 2019
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Does low fluorophore pKa = low pH sensitivity?
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2
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2104
|
August 18, 2019
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New spectra viewer available on FPbase
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0
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1069
|
July 11, 2019
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