“…1, 0.5 and 0.2 μm beads are TetraSpeck Microspheres from Life Technologies…”
from A line scanning confocal fluorescent microscope using a CMOS rolling shutter as an adjustable aperture
https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2818.2012.03642.x
Some years ago (2013 or 2014), the author visited my previous company to give a talk. In my previous company we would use ~100nm beads for a more conventional confocal. They are using 200nm beads as the smallest. Recently (2017) in my current lab, I put together something similar on an optics table, using generic beads found on Taobao or beads made by another lab in the material science department. Unlike other “slit” or “line” scanning setups, it is a variable “slit.” Normally, a “slit” or a pair (excitation/detection) would be fixed and terminology such as “line spread function” would be employed to characterize resolution (derive the MTF) as a cylindrical lens is often used (with the intensity unevenly distributed, with better resolution in the middle and worse at the edges, similar to some light sheet) Alternatively a “line” of fixed pinholes may be used as in “swept-field” confocal. Usually in a “swept-field” they have several sets of “lines” of pinholes, with each set of different diameters, for excitation and detection, which can be quickly changed using a piezo.
Lateral and axial resolution is not really that great in any of these systems compared to more conventional confocal approaches, however you do get some optical sectioning ability. Apologize for the quotation marks, as if you have read a lot of the different papers about these slit/line approaches, the terminology/jargon gets a bit confusing. Likewise, the different papers have different (sometimes dubious) methods to characterize resolution.
In the INCELL it will depend on the settings in the software which will determine the width of the “aperture” (the width of the rolling shutter). Did you call Cytiva (Molecular Devices) to ask them? The service engineer who installed or maintains it could perhaps explain to you how they QC it on-site to verify its performing properly. Something such as that is probably not in the user manual, but the engineer has to have a standard sample or SOP to prepare one. Its was quite a pricey piece of equipment so I would expect they may be able to help if you ask.
Also, some of the machines Thermo sells employ a similar mechanism as the INCELL, using the same patent under license.
