mTurquoise2, mNeonGreen and mCherry in Yeast

Probes & Prep
1

Hi everybody,
Has anyone ever successfully expressed mTurquoise2, mNeonGreen and mCherry in Yeast? Was there a lot of codon optimization? Any reason why we should not go for these tags in our future yeast experiments?
Thanks and best regards,
Laurent.

2

Have you seen this article?

https://www.nature.com/articles/s41598-019-38913-z

Hard to find a better characterization than that.

3 Likes
3

Hi Talley,

Thanks a lot, I had totally forgot about this one!

Indeed, it will be difficult to do better
J

Very best regards,

Laurent.

4

I was involved in that study - let me know if you have questions!

2 Likes
5

Hi Joachim,

Thanks, I’ll do!

Best,

Laurent.

6

Definitely have imaged Cherry for a user, it was dim-ish and liked to bleach, but played with imaging conditions and was able to image about 20 minutes or a bit longer with no photobleaching. I have no experience with the others in yeast. Good luck! BTW - mRuby seemed better in yeast than Cherry.