Does anyone have recommendations for a blue fluorescent protein that’ll work well for SIM (specifically with the OMX)?
Apparently a while ago a GE rep suggested TagBFP. A user spent months going through the whole process of tagging a lamin with it before chatting with me and seeing how poorly matched the OMX’s DAPI emission filters are to TagBFP. Looking at the FPbase efficiency report fills me with despair.
Her cells have been selected by FACS, so it’s not an issue of continuously selecting cells with weak TagBFP expression.
Thank you for any recommendations.