Fluorescent nanobodies that target GFP?

Probes & Prep
1

Hi all, I’m looking for anti GFP fluorescent nanobodies conjugated with Alexa 647 (or equivalent) for immunostaining of samples with GFP in order to do dSTORM imaging. Does anyone know of any commercially available fluorescent nanobodies that target GFP?

So far I have found the NanoTag FluoTag options and ChromoTek GFP-boosters.

2

Hi, you already found the best GFP nanobodies to use for dSTORM.
The FluoTag®-X4 anti-GFP - Alexa647 works quiet well.
Look up the publication that validated these GFP nanobodies for labeling efficiency:
Thevathasan et al., 2019 Nat Methods.