My lab and another lab have observed that staining MDCK and MEF cells with CellMask membrane stain (Deep Red version, if it makes any difference) causes the cells to round and, in some cases, eventually detach. I was wondering if anyone else has noticed something similar.
We’re using the 0.5x - 1.5x concentrations recommended by the product sheet and 5 min incubations at 37degC. CellMask isn’t killing the cells because the cells will reattach and spread if introduced to another substrate. We haven’t tested any other cell types. It doesn’t matter whether the cells are attached to a cell culture dish or collagen-coated or Fn-coated glass.