I am currently working on measuring the PSF of the confocal microscope, and then I will use that PSF to deconvolve an image. However, I am not certain with the sampling distance I use.
My equipment are listed below:
-0.1 micrometer latex beads(to measure the PSF)
-473nm light source
-100x,0.8NA Air lens
-Piezo stage to scan the specimen
-Single-photon avalanche diode as the detector
I estimate the resolution of my microscope to be 360nm by using 0.61λ/2NA formula, in order to satisfy the Nyquist theorem, I divide 360nm by 3 which equals to 120nm. So I set 120nm to be the sample the PSF of the latex beads, but I am not sure whether 120nm is small enough to sample the PSF of 0.1micrometer latex beads and deconvolve an image. Is there any suggested article or paper discussing about this problem?
Thank you all for reading.