Mounting media and fluorophore compatibility

Hi,

Some report that some mounting media are not compatible with some fluorophores.

For example possibly Vectashield is not compatible with far red fluorophores. Is this correct ? or is this specific possibly to only one type of far red fluorophores eg Alexa Fluor but nor Atto ?

Are there other known compatibility issues between mounting media and fluorophores or FPs ?

Thanks a lot

The compatibility between mounting media and fluorophore is generally due to the anti-photobleaching/antifade reagent used. Each anti-photobleaching reagent works well for some fluorophores, but may quench or not work as well for other fluorophore types.

Any company that makes mounting media should provide a fluorophore compatibility table somewhere in the product literature; for Vectashield, there’s one here (scroll down).

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