Hello everybodies,
Do you know if medium or phenol red can have an influence on fluorescence or second harmonic or third harmonic generation??
kind regards
Hi @Alex.h,
I never did SHG/THG, but as for fluorescence - yes, media might have an influence, but it depends… For conventional DMEM w/ phenol red I never have an issue to track a cell cycle progression using mCherry fluorescence as a reporter. But in this case I didn’t care much about precision. However, for the FRET measurement with CFP/YFP pair I switched to FluoroBrite DMEM (proprietary media from ThermoFisher) because DMEM, even phenol red free, gave me too much background.
Maybe you already found the answer for SHG/THG elsewhere, if so, please share)
Best,
Oleg