Hello everybody, and thank you all for creating such useful communities on image analysis world.
I am a cell biologist and I’m approaching to image acquisition and analysis automation on fixed and living samples.
In my former lab, to automate the selection and the acquisition of specific field or phenotype, I used successfully the Nikon NIS software with JOBS module with a Nikon Ti eclipse microscope.
Now, I have to put on a brand new system in a new lab and I’m wondering if there are other comparable and maybe freeware options.
What I’m interesting in is a microscope with a software able to create acquisition pipelines (preferably with user friendly interface).
To better explain what I’m looking for, with Nikon JOBS I could create a pipeline like this:
Selection of 20x objective
Selection of acquisition area size
Autofocus on dapi
Acquisition and analysis to look for mitotic cells (based on dapi signal)
If mitotic cells are in the field:
- selection of 63x objective
- acquisition with z-step on multiple lambda channels
If mitotic cells are not in the field:
- move to next field
Do you have experience and/or advice on alternative Microscope/Software combination?
I think that uManager could be a good freeware option but I’m concerned about compatibility with the microscope and on the time I will consume learning code for pipelines creation.
Sorry for the probably naive issue!
Thanks a lot!