Extra information:
- I used Fiji 2.1.0/1.53c Java 1.8.0_172 (64 bit)
- see enclosed a snapshot of the image display in Fiji (Multi-positions acquisition, 2 channels, Zstack)
- the associated json file as text below.
{
“encoding”: “UTF-8”,
“format”: “Micro-Manager Property Map”,
“major_version”: 2,
“minor_version”: 0,
“map”: {
“AutoscaleIgnoredQuantile”: {
“type”: “DOUBLE”,
“scalar”: 0.0
},
“Autostretch”: {
“type”: “BOOLEAN”,
“scalar”: true
},
“ChannelSettings”: {
“type”: “PROPERTY_MAP”,
“array”: [
{
“Channel”: {
“type”: “STRING”,
“scalar”: “Filter A - DAPI”
},
“ChannelGroup”: {
“type”: “STRING”,
“scalar”: “4- Fluorescence”
},
“Color”: {
“type”: “COLOR”,
“scalar”: {
“ColorSpace”: “sRGB”,
“Components”: [
0.0,
0.0,
0.8
],
“Alpha”: 1.0
}
},
“ComponentSettings”: {
“type”: “PROPERTY_MAP”,
“array”: [
{
“Gamma”: {
“type”: “DOUBLE”,
“scalar”: 1.0
},
“ScalingMax”: {
“type”: “LONG”,
“scalar”: 255
},
“ScalingMin”: {
“type”: “LONG”,
“scalar”: 0
}
},
{
“Gamma”: {
“type”: “DOUBLE”,
“scalar”: 1.0
},
“ScalingMax”: {
“type”: “LONG”,
“scalar”: 9223372036854775807
},
“ScalingMin”: {
“type”: “LONG”,
“scalar”: 0
}
}
]
},
“HistogramBitDepth”: {
“type”: “INTEGER”,
“scalar”: 16
},
“UniformComponentScaling”: {
“type”: “BOOLEAN”,
“scalar”: false
},
“UseCameraBitDepth”: {
“type”: “BOOLEAN”,
“scalar”: true
},
“Visible”: {
“type”: “BOOLEAN”,
“scalar”: true
}
},
{
“Channel”: {
“type”: “STRING”,
“scalar”: “Filter N21 - TRITC”
},
“ChannelGroup”: {
“type”: “STRING”,
“scalar”: “4- Fluorescence”
},
“Color”: {
“type”: “COLOR”,
“scalar”: {
“ColorSpace”: “sRGB”,
“Components”: [
0.8,
0.0,
0.0
],
“Alpha”: 1.0
}
},
“ComponentSettings”: {
“type”: “PROPERTY_MAP”,
“array”: [
{
“Gamma”: {
“type”: “DOUBLE”,
“scalar”: 1.0
},
“ScalingMax”: {
“type”: “LONG”,
“scalar”: 256
},
“ScalingMin”: {
“type”: “LONG”,
“scalar”: 0
}
},
{
“Gamma”: {
“type”: “DOUBLE”,
“scalar”: 1.0
},
“ScalingMax”: {
“type”: “LONG”,
“scalar”: 9223372036854775807
},
“ScalingMin”: {
“type”: “LONG”,
“scalar”: 0
}
}
]
},
“HistogramBitDepth”: {
“type”: “INTEGER”,
“scalar”: 16
},
“UniformComponentScaling”: {
“type”: “BOOLEAN”,
“scalar”: false
},
“UseCameraBitDepth”: {
“type”: “BOOLEAN”,
“scalar”: true
},
“Visible”: {
“type”: “BOOLEAN”,
“scalar”: true
}
},
{
“Channel”: {
“type”: “STRING”,
“scalar”: “Filter I3 - FITC”
},
“ChannelGroup”: {
“type”: “STRING”,
“scalar”: “4- Fluorescence”
},
“Color”: {
“type”: “COLOR”,
“scalar”: {
“ColorSpace”: “sRGB”,
“Components”: [
0.0,
0.8,
0.2
],
“Alpha”: 1.0
}
},
“ComponentSettings”: {
“type”: “PROPERTY_MAP”,
“array”: [
{
“Gamma”: {
“type”: “DOUBLE”,
“scalar”: 1.0
},
“ScalingMax”: {
“type”: “LONG”,
“scalar”: 256
},
“ScalingMin”: {
“type”: “LONG”,
“scalar”: 0
}
},
{
“Gamma”: {
“type”: “DOUBLE”,
“scalar”: 1.0
},
“ScalingMax”: {
“type”: “LONG”,
“scalar”: 9223372036854775807
},
“ScalingMin”: {
“type”: “LONG”,
“scalar”: 0
}
}
]
},
“HistogramBitDepth”: {
“type”: “INTEGER”,
“scalar”: 8
},
“UniformComponentScaling”: {
“type”: “BOOLEAN”,
“scalar”: false
},
“UseCameraBitDepth”: {
“type”: “BOOLEAN”,
“scalar”: true
},
“Visible”: {
“type”: “BOOLEAN”,
“scalar”: true
}
},
{
“Channel”: {
“type”: “STRING”,
“scalar”: “Filter N21 - TRITC”
},
“ChannelGroup”: {
“type”: “STRING”,
“scalar”: “4- Fluorescence”
},
“Color”: {
“type”: “COLOR”,
“scalar”: {
“ColorSpace”: “sRGB”,
“Components”: [
0.8,
0.0,
0.0
],
“Alpha”: 1.0
}
},
“ComponentSettings”: {
“type”: “PROPERTY_MAP”,
“array”: [
{
“Gamma”: {
“type”: “DOUBLE”,
“scalar”: 1.0
},
“ScalingMax”: {
“type”: “LONG”,
“scalar”: 99
},
“ScalingMin”: {
“type”: “LONG”,
“scalar”: 0
}
},
{
“Gamma”: {
“type”: “DOUBLE”,
“scalar”: 1.0
},
“ScalingMax”: {
“type”: “LONG”,
“scalar”: 9223372036854775807
},
“ScalingMin”: {
“type”: “LONG”,
“scalar”: 0
}
}
]
},
“HistogramBitDepth”: {
“type”: “INTEGER”,
“scalar”: 16
},
“UniformComponentScaling”: {
“type”: “BOOLEAN”,
“scalar”: false
},
“UseCameraBitDepth”: {
“type”: “BOOLEAN”,
“scalar”: true
},
“Visible”: {
“type”: “BOOLEAN”,
“scalar”: true
}
}
]
},
“ColorMode”: {
“type”: “STRING”,
“scalar”: “COMPOSITE”
},
“PlaybackFPS”: {
“type”: “DOUBLE”,
“scalar”: 10.0
},
“ROIAutoscale”: {
“type”: “BOOLEAN”,
“scalar”: true
},
“UniformChannelScaling”: {
“type”: “BOOLEAN”,
“scalar”: false
},
“ZoomRatio”: {
“type”: “DOUBLE”,
“scalar”: 0.3333333333333333
}
}
}