I’m looking for an efficient system to induce heat shock (45 degrees) on plant organs (meristems) placed in petri dishes and observed under an upright confocal microscope. I need to image whilde inducing the heat shock (from RT to 45°C, not necessary very fast)
In practice the organs are inserted in agarose 1% in in a petri dish and covered by liquid culture medium, the imaging is done using an immersion objective.
I’m wondering if it would be efficient to use a perfusion system combined with a temperature controller to change the temperature or if a petri dish heater would be sufficient?
Does anybody have ever used this kind of system: Open heated perfusion chamber for Water Immersion Objectives of upright and long working distance objectives of inverted microscopes, TC-WI, temperature controlled microscope stages with a temperature controller for example to do a heat shock while imaging.
Thanks a lot in advance for you tips or suggestions,