Cell counting with ImageJ

I was trying to do cell counting analysis on some of my cultured rat astrocytes whose boundaries are quite indistinct as the cells are flat and confluent, by watershed after having done some adjustment to the images. The main problem I think I have here is that the colour intensity of the “background” is almost the same as that in the “cell body” on the images. How should I approach it?

Hi Katie. This forum focuses on image acquisition. The analysis experts (including many of the ImageJ developers) are on the image.sc forum. You should ask your question there.


Hi Jennifer,
I see - thank you so much!

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