Does anyone have experience with expansion microscopy on lamina ?
I tried the protocol from Gambarotto, D.et al. (2018). Imaging cellular ultrastructures using expansion microscopy (U-ExM). Nature Methods . https://www.nature.com/articles/s41592-018-0238-1
Below is with methanol fixation. I tried without fixation and after immunostaining I couldn’t see anything.
Below and image of an expanded sample
But in reality it should look more like this (below a non expanded sample - not the same used for expansion)
Does anyone have any ideas on how to improve this ?