Coupling lasers to a widefield microscope?

[Hazen_Babcock]

This thread has some discussion of how to align a laser into a microscope:

Basic microscopy Questions about building

Hello everyone, Ive recently been tasked with building an inverted objective-tirf microscope. Im rather new to this so im learning by doing. I have built the system and aligned the laser, as far as I know. After aligning everything and connecting the cameras and hardware, I decided to do some test images. However, these images are a little strange. Perhaps you could help me out. The images were taken in the epifluorescence position (e. g the laser shining directly through the sample) with just …