Dear µforum community,
I’m considering the following setup on a Nikon Eclipse Ti microscope with a Nikon TIRF unit and would appreciate your advice on feasibility:
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Convert the 3 mm LLG input into an FC/APC input using Thorlabs components (links below).
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Attach a 2 W, 642 nm single-mode laser (FC/APC fiber, model 2RU-VFL-P-2000-642-B1R-FCAPC from MPC Communications) to this newly modified input.
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Keep the CellX for Microscopy laser engine (4 × 100 mW at 405, 488, 561, and 637 nm from Coherent, also single-mode with an FC/APC fiber) connected to the original FC/APC input on the Nikon TIRF unit.
Please see the attached diagram for reference.
In order to determine whether this configuration will work, I’d also like to better understand the optical path inside the Epi-Fl illuminator. Could you please share any information regarding its collimator and other internal components?
Thank you for your time, and I look forward to your insights.
Warmly,
Yossi
Relevant Thorlabs parts:
• SM2A6
• SM2N3
• AD11F
