I’m suffering from a very weak signal of yelow dye, such as CF568 and JF549, in my dSTORM imaging. I used yellow dye-conjugated Halo tag or antibody to stain my samples. Althrough I can see a very bright and specific staining under TIRF or confocal microscope, the brightness of localizations under dSTORM is very weak and make it impossible to identify a single molecules. Does anyone has an experience on that? Any suggestions will be highly appreciated.
Attached is an example of my dSTORM image.