I’m trying to learn to do DNA-PAINT following the Jungmann Nature Protocols paper. I’m using Ibidi 6 well flow chambers. I’ve noticed that the imager strand conjugated to Cy5 is very sticky and creates a lot of background, while a similar sequence with Cy3 does not.
Does anyone have recommendations for cleaning the slide or changes to the buffer that would prevent Cy5 from sticking to the glass? Since I want to use these pre-made chambers for convenience, I don’t think plasma cleaning will be feasible (nor do I have that equipment).