Dear all,
I was wondering if you know what the gain in the HyD detector is doing (for instance on the Leica laser scanning confocals)?
My feeling is that it is something like a digital gain. This means a factor used in the conversion from the analog (photon counting) to the digital signal (your image).
I am always a little worry to oversaturate/break the detector and I am not sure where this is occurring.
On the Leica SP8 if you set the HyD-detector to photon counting mode the gain is at 10%. Is this the true signal detected without digital gain?
Thanks
Antonio