I recently watched the excellent training video for the LSM880 by Jason Kirk for reference of our own facility. In his video he mentions using the Digital Offset slider for the PMT to eliminate “blue” (underexposed?) pixels. I always thought that I should not touch this setting, as I was afraid I would be cutting off “real” background pixels, that would better be subtracted during post-processing.
A previous thread discussed this a bit, but largely focused on saturation. I’d like to open up the discussion on “what should be done” for underexposed pixels. Is it bad to leave pixels underexposed for quantification later?