Hello, everyone.
I am trying to perfuse different drugs onto my sensors to characterise my sensors. I realise whenever I change to another reservior during the imaging, there is an artifect (I can see this with GFP only cells) in both high and low flow rates.
Does anyone know how can I eliminate this? I couldnt find documentations that describe very detailed methods for setting up the perfusion system.
I was hoping that I can get some insights if anyone tried similar experiment.
Nikon Ti2
-Perfusion system: VCPlus-4 Channel Gravity Systems - ALA Scientific
I tried few different chambers:
-Chamber(MS-512sp):Round Chamber for 12mm Round #1 or #2 Cover Glass - ALA Scientific
-Chamber(RC-26): RC-26 / RC-26G - Open Diamond Bath Imaging Chambers | Warner Instruments
-chamber (MS-518SP):Oval Chamber for 25mm Round #1 or #2 Cover Glass - ALA Scientific
