Oil with RI of water for use with Olympus water objective

Dear all,

I have a 60X water immersion Olympus objective that I’d like to use for prolonged imaging. Thus, water is not an option for the immersion media, as it will evaporate over the course of the experiment. Olympus does not sell any oil with the RI of water (in contrast, Zeiss does for its W objectives), so I was wondering if anyone had experience with this.

Specifically:

  1. Are there particular water-RI oils that you know are (or aren’t) compatible with Olympus objectives?
  2. Are there particular water-RI oils that have lower fluorescence, convenient viscosity, and/or low dispersion, and would recommend?

The obvious options are Cargille and the Zeiss oil, any of which I would verify are compatible with Olympus before using on the objective. I was hoping the collective knowledge on the list would narrow my options down to a few good candidates.

Thanks in advance.

Pablo

I use Zeiss Immersol W on an Olympus water immersion objective (bottom objective on a lattice) for months without changing it. Granted, it’s not a high NA lens, and we’re not asking much of the image quality there (and it’s a real pain to get to it to replace the media). I think many other people have also used Immersol across objective brands. So, i would certainly keep that on your list of options (but, as you said, do check with olympus just to be safe).

Thanks for the suggestion Talley.
I’ve asked Olympus whether they consider the Zeiss Immersol W to be safe on their objectives.
I’ll update when I get their answer.

I think Cargille also sells an oil with RI 1.33. I can’t seem to find it on the mobile-optimised site, but the home page mentions it. Might be worth checking the non-mobile site.

For medium time frames (5-8hrs w stage to heater) we have had good luck with GenTeal, an aqueous gel for eye dryness.

Thanks for those suggestions Wendy.

We have two types of Cargille oils with RI 1.33 at room temp and at 37C.

Our 20x WI objective has a very large working distance, and the oils are not viscous enough to allow contact with the sample.

I’ll echo Wendy’s statements regarding GenTeal.

Thanks for the information.
Luckily this is for a 60X W, with a much shorter working distance than your 20X, so contact should not be an issue.
With respect to the GenTeal, any problems with impurities that cause autofluorescence or reduce quality?

I haven’t done a hard core test–went with the assumption that if it’s intended to go in a human’s eye it’s probably pretty clean. Certainly nothing completely egregious. We were using it with 40X and 25X WI w 120-500um WD.

I think it costs ~$5 at your local drug store so it’s low risk to test!