I have a 60X water immersion Olympus objective that I’d like to use for prolonged imaging. Thus, water is not an option for the immersion media, as it will evaporate over the course of the experiment. Olympus does not sell any oil with the RI of water (in contrast, Zeiss does for its W objectives), so I was wondering if anyone had experience with this.
- Are there particular water-RI oils that you know are (or aren’t) compatible with Olympus objectives?
- Are there particular water-RI oils that have lower fluorescence, convenient viscosity, and/or low dispersion, and would recommend?
The obvious options are Cargille and the Zeiss oil, any of which I would verify are compatible with Olympus before using on the objective. I was hoping the collective knowledge on the list would narrow my options down to a few good candidates.
Thanks in advance.