Is it recommended to use an objective such as the Nikon CFI Plan Fluor 40x Oil /1.30 (https://www.microscope.healthcare.nikon.com/selectors/objective-comparison/-1829) for long-term live cell imaging at 37 degrees?
My gut says no because there’s no correction collar for temperature (hello aberrations). One other concern is if the objective warmer is potentially harming glue or something else within the objective.
(You may assume it’s a thin adherent sample on the coverglass)
Thank you all in advance.