In my quest to build the cheapest high-end spinning disk microscope I ended up demoing a Leica Thunder widefield system in my lab. A few people wrote to me asking for my impressions so I will share them. While I’m under no illusion that a widefield could replace a confocal I was curious what Leica w…

Thanks to the guys at Leica, I could also try out the new-ish Hamamatsu Fusion. The Fusion is supposedly a completely new sensor and is not made by Fairchild like the 4.2 sensor found in many sCMOS cameras. I couldn’t find any information about who actually produces the sensor for Hamamatsu that go …

Thanks for taking the time to put all of these posts together. I have really liked the wide area “click into” feature on the Zen software, so I am glad to hear it will also be on the Leica systems. [image] Andrew_Seeber: One give away is the “banding” around the edge of the nucleus. As with …

Thanks for the great comparison, Andrew! For the ‘Thunder’, is it just on/off or can you control any parameters at all? The results you show reminded me of what you get using the ‘Dehaze’ feature available in Adobe Lightroom, so I applied that to your figure. The left column is widefield, upper rig…

Thank you Andrew! For such detailed comparison! We are in the process of demoing Andor Dragonfly 500. I will also put some updates here!

ORCA Fusion looks great, I’ve always had great success with Hamamatsu sensors! Regarding Thunder, I’ve seen it on 2 Leica Systems, a high-end system and a fluorescent stereoscope, I think it was promising, but seemed ‘heavy handed’ when trying it on the stereoscope with different samples. You’re rig…

Hi meatball, I’d love to see how the Dragonfly compares. That would be great Neil

@JamesOrth , can you elaborate on what you mean by heavy handed for the stereoscope? We’ve seen some Thunder data from an inverted system and it looked great. For reference, we us Microvolution for all our decon and works really well. Thunder separates the signal into ‘in focus’ and ‘out of focus’…

Perhaps it was the sample or imaging parameters that contribute to the issue, but the images looked over-processed, dark edges around objects, etc., and some artificial structure I kept talking about tweaking the parameters but it didn’t happen, the “recommended settings” were being used. And I co…

That’s very interesting about lightroom. The effect is very similar :face_with_monocle: I got some more info on what Thunder was actually doing: Below is a screengrab of the settings page for Thunder, you can change what they call the feature scale and you can change strength. [Overview] As I un…

Hi @Andrew_Seeber ! Thanks for sharing your impressions. Your last post caught my eye, as those settings and results look really similar to those of an unsharp mask filter, like the one available in ImageJ (and Fiji). Here’s a quick comparison. I converted the images from the post to 16-bit before…

Leica Thunder and a comparison between 4.2 cmos versus Hamamatsu ORCA-Fusion

Hardware

Andrew_Seeber October 15, 2019, 3:02pm 15

@talley Good point about that white paper. Leica shared it with me; doesn’t look like we can add .pdf attachments to this forum so I’ll share the relevant page.

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Leica Thunder and a comparison between 4.2 cmos versus Hamamatsu ORCA-Fusion

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