Carlos is actually building the microscope based on our plans :), he just didn’t mention it ;)
to answer one of the questions: this lightsheet system doesn’t scan optically because it’s very simple, so global shutter GS is just enough in the first approach, unless a rolling shutter RS would somehow intrinsically improve contrast and SNR at the same price tag of a GS camera (but here I am not fully aware of RS improving image quality over GS without playing on the illumination scheme).
The main goal of Carlos is to look for a “well behaving” camera (acceptable sensitivity, good SNR, hence low noise, good dynamic range minimum 12bits, very light footprint and no specific cooling system) for a price tag below or similar to the one we have tested (see above), i.e. about 650EUR.
Our decision to take this camera from Imaging Source was empirical and simple:
- it was suggested by colleagues (Francesco Pampaloni et al) at Univ. Frankfurt who used it for their cost effective SPIM-DSLM system, hence we could test it without buying it thanks to them,
- it behaved much better (more sensitive, better SNR) than its Thorlabs CMOS (DCC3240M) equivalent that we had originally bought, but it was much cheaper.
Hence the key aspect that Carlos is trying to sort out is, indeed, how can we anticipate on the sensitivity of industrial cameras for fluorescence applications, when most of the time the manufacturers don’t really provide specs the enable one to make a good guess (quantum efficiency etc… usually missing from specs), and without having to benchmark too many models (which turns out to be time-consuming, or just expensive).
Further note: the lightsheet system Carlos develops needs a TTL-triggerable camera.
Thanks in advance to everyone for helping Carlos !