Leica M205A and MicroManager

You will have to see if your microscope came with any documentation on how to use an API they may have provided. I haven’t worked with Leica microscopes but I have used Zeiss MTB API which has great documentation. If nothing else works you could use Windows Automation functions to move the focus since you already can control the camera you are one step closer to fully controlling acquisition. Let me know if your microscope came with a .NET API like Zeiss does and I will be able to help. I have made .NET projects which may be of use incase Leica provided a .NET API. GitHub - BiologyTools/BioImager: A .NET microscopy imaging application based on Bio library. Supports various microscopes by using imported libraries & GUI automation. Supports XInput game controllers to move stage, take images, run ImageJ macros on images or Bio C# scripts. and GitHub - BiologyTools/BioCore: Bio Library in .NET6. Bio is a library & program for annotating, & editing various microscopy imaging formats using Bioformats supported images. including whole slide, pyramidal & series.