We want to switch to LED light sources for epifluorescence for several of our systems. The SOLA SE U-nIR looks very interesting, because it can also excite Cy7, for a moderate cost increase when compared to the no nIR model. We thus had the idea of buying a few interchangeable and identical light sources which could all excite Cy7.
However, in these range of wavelengths, not all filters have OD>6. So what’s your opinion ? Is there a significant risk of nIR excitation bleedthrough when using this light source in combination ‘standard’ filter cubes for DAPI, GFP etc.?