I want to perform immunocytochemistry on transfected HEK cells. I’ve been getting them to grow and adhere to Ibidi 12 well glass slides while they’re growing. I can do media changes and they stay. But once I take the out and fix and then do some washes, they are mostly gone. it’s frustrating. The glass is coated with PDL for 2 hours before seeding the cells.
Does anyone have tricks for getting HEK cells to stay on the glass for ICC?