Hello everybody,
Does anybody know a good resource describing the effect of multimerization (dimerization) on the lifetime of a fluorophore. Something like the theory or the measurement of the lifetime of GFP vs a dimer of GFP in solution ? (no FRET, just a single fluorophore / FP being multimerized.)
Thanks!
This paper (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770629/pdf/main.pdf) states:
“Because homo-FRET concerns energy transfer between identical fluorophores, it does not affect the emission spectrum or the fluorescence lifetime of the probes.”
This is what I’ve heard from the experts in the field as well. Perhaps there’s some proof in older work. I wouldn’t be surprised if this is explained in “Principles of fluorescence spectroscopy” by Joseph R. Lakowicz (but I don’t have a copy at home, so I cannot check it).
Perhaps these references are useful:
https://www.nature.com/articles/srep33022
https://dx.doi.org/10.1016%2FS0006-3495(01)76265-0