Confocal Microscopy Dichroic mirror

Theory
1

Confocal microscopy dichroic mirror passes two rays to opposite directions (from the laser and specimen)

Does this two-way traffic cause problems/ interference? According to theory, it works fine. Can anyone explain how do two-way rays work without any problem?

By the way, I have never used any confocal microscope.

Thanks in advance

2

There is no interference. The fluorescence emitted by the specimen and the excitation laser light are not coherent and they have different wavelengths (due to the Stokes shift), so these photons cannot interact.

3 Likes
3

Thank you for the clarification.