Does 2x scan speed + 2x averaging reduce photobleaching compared to 1x scan speed + 1x averaging (no averaging)?

Hey @JelleP,

This general question of how illumination regime relates to photobleaching is still one with rather little hard empirical data behind it, but opinions on the topic are absolutely repeated all the time. To make matters worse, the few papers that have delved into very specific aspects of the photophysics here have often been referenced poorly and/or out of context. suffice it to say, it’s a super complicated topic, and I think most answers you’ll hear on this topic are largely folksy opinions stemming from uncontrolled observations.

I gave a longer response to this question in a previous issue:

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