LHello Everyone,
I am currently using fluorescent tetraspeck beads for image registration in lightsheet multiangle microscopy. However, I’ve noticed that these beads display a notably weak signal in the DAPI channel, which has complicated the process of aligning nuclei channel to other channels.
I’m looking for alternatives to these beads that would yield a stronger signal intensity in the DAPI channel. I even use just plain mirror instead of filter to capture all wavelengths from excitation at 405 nm. Has anyone had a similar issue and found a suitable replacement? This could be a different type of bead or perhaps a different method altogether that is compatible with lightsheet microscopy.
Any suggestions, experiences, or advice you could share would be greatly appreciated.
Thank you for your time and I eagerly await your responses.
Best regards.