Transwell migration imaging

A user in our facility wants to do live imaging of cell migrating through a transwell. Does anyone has any tips on how to set-up the assay?
Thanks! :slight_smile:

This sounds tricky given the typical orientation of a transwell device. Depending on the size of the device and material the cells are migrating through, it may be possible to use a long working distance objective and perform z-series through the thickness of the well, as long as there isn’t too much autofluorescence of the material the cells are migrating through. This sounds like a fun thing to try, I hope it works for you.