We are working on an application requiring rapid stage translation between multiple fields of view (FOVs). Recently, we got an interesting suggestion from a reviewer, asking us to “comment on the tolerable speed in lateral and axial directions” from the perspective of drag resistance between the immersion medium and the coverslip.
I thought maybe some of you have thought about this in more depth and for longer, so I am curious about any insights you might have?
Of course, any information that would contribute to our response to the reviewer would be properly acknowledged!
Briefly, here is what I’ve come up so far:
- intuitively I would think that the translation is less affected in the axial compared to the lateral direction
- drag should be proportional to the viscosity of the immersion medium, such that lower viscosity results in less drag
- if the timescales of the drag and viscous behaviour of the immersion medium are much lower than the stage response and translation timescales, then there is a separation of timescales and we can ignore the effect of drag for practical applications
Looking forward to hearing your thoughts!