Practicing and trial and error with the agarose and beads regardless of application is time consuming. There are many protocols, which skip smalls details learned from making mistakes. As @Emmanuel_Reynaud mention you get one small thing wrong…it can become frustrating, especially when you are “almost” finished with a custom system trying to determine what’s. When you finally get it right the sample that gives you slightly better results…it doesn’t last very long and you have to make another. Some years ago, the local distributor for a company called Argolight let me borrow a test slide…
There are some limitations with their standard slides regarding water. However, I am wondering if anyone has tried their custom offering for Lightsheet/SPIM?