Could someone kindly help me understand the implication of the intensity profile plot between two different tags? E.g EGFP for protein X and mCherry for protein Y. Thanks. Remi.

If it is a line plot, you are usually looking at quasi-colocalization in terms of peaks overlapping at the same locations along time line. This does not mean that the proteins are interacting in any way, but can give you an idea if they are associated. Two general cytoplasmic proteins could look ver…

@Research_Associate , Thanks very much for your reply. Yes, it’s a line plot. With reference to this quasi-colocalization, can I, for example, count the number of peaks in line A and line B, and then infer this quasi-colocalization from the number of overlapping peaks? So, in what situation might a…

While it is pretty much impossible to prove interaction with a plot like that, it is surprisingly easy to show that two proteins are almost never in the same location at the same time. Or if you want to show that one localizes to the same or different compartments as a third marker (DAPI, MitoTracke…

Yes, I also recommend you bring this question to the image analysis forum.

Meaning and implication of intensity profile plot

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Research_Associate August 12, 2019, 3:52am 5

Also, as this is less of a microscope question, and more of an analysis question, you might get better or more informative responses on the analysis forum. Similar posts have also been diverted.

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Meaning and implication of intensity profile plot

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