EMCCD vs BT sCMOS dragonfly dual camera

Hello, we’re looking into a dragonfly 2camera system with SRRF and TIRF.

Typical config is Ixion 888 EMCCD + Zyla sCMOS. But with the Sona BT 95% QE it seems this could make the ixion 888 defunct and not worth having on there and that 2x SONA is the way to go.
The SONA also has SRRF built in as a bonus.

Am I unfairly discounting the EMCCD and assuming it will be defunct in 5-10 yrs time?

We typically image dim proteins in fungi and bright dyes in host/pathogen for 4D capture.

Many thanks for any input!


Darren Thomson

There is QE and there is shot noise. EMCCDs amplify before A-to-D readout so will have a lower floor of detection. But they come with the risk of burn damage if you are also doing bright TIRF studies. I have owned three EMCCD cameras and all were problematic. So if you can see what you need to, the sCMOS will probably be much more reliable. You may have too high a background from the spinning disc to make good use of the EMCCD low end anyway, which was true on CARV systems. Yokogawas see some benefit from electron multiplication, if you have the funds to repair, or only have very careful users.