96-well plates you would recommend for high NA imaging

Hello everybody,

I’d like to know if you have some recommendations for 96-well plates (well not necessarily 96) that would be suitable for high-NA imaging.

Ideally, the well plate would:

  • have a very homogeneous thickness, of the order 170 - 190 um
  • have no skirt, thus allowing to focus all wells with high-NA objectives
  • be reasonably cheap

The well-plates we recommend at our facility is the PhenoPlate 96 from Perkin-Elmer, but we would like to know alternative options.

Thanks!

Nicolas

(this will be crossposted on the confocal list)

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Hello,
we use often those
https://zell-kontakt.de/products/imaging-plate/imaging-plate-glasscover-2/

They are fine. I normally avoid to image the outer wells as it get quite tight. It depends on how bulky your objective is.

Antonio

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You may have had other replies, we’ve had good luck with 96 well plates from CellVis, they use 1 piece of #1.5H German glass and are well priced. They do have a skirt so you likely can’t image the entire plate with high-NA low WD lenses.

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https://www.auroramicroplates.com/#products

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Hi everybody,

Thank you for your answers! I’ve made a google sheet with the suggestions coming from this forum and with the ones from the confocal list:

If you want to edit it to add some more, please let me know and I’ll give you edit rights.

Nicolas

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Thanks for compiling the list! We also have good experience with
Corning 4580 half area glass bottom plates if you’d like to add

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Thanks, I’ve added it, but I couldn’t find the skirt size unfortunately

Details can be found here

Skirt Height (mm) 6.096

Here additional dimensions, unfortunately they do not provide a drawing