I’d like to know if you have some recommendations for 96-well plates (well not necessarily 96) that would be suitable for high-NA imaging.
Ideally, the well plate would:
have a very homogeneous thickness, of the order 170 - 190 um
have no skirt, thus allowing to focus all wells with high-NA objectives
be reasonably cheap
The well-plates we recommend at our facility is the
PhenoPlate 96 from Perkin-Elmer, but we would like to know alternative options.
(this will be crossposted on the confocal list)
we use often those
They are fine. I normally avoid to image the outer wells as it get quite tight. It depends on how bulky your objective is.
You may have had other replies, we’ve had good luck with 96 well plates from CellVis, they use 1 piece of
#1.5H German glass and are well priced. They do have a skirt so you likely can’t image the entire plate with high-NA low WD lenses.
Thank you for your answers! I’ve made a google sheet with the suggestions coming from this forum and with the ones from the confocal list:
The well plates listed should be compatible with high-NA imaging, meaning:
- have a very homogeneous bottom thickness, of the order 170 - 190 um
- have no skirt, thus allowing to focus all wells with high-NA objectives
If you want to edit it to add some more, please let me know and I’ll give you edit rights.
Thanks for compiling the list! We also have good experience with
Corning 4580 half area glass bottom plates if you’d like to add
Thanks, I’ve added it, but I couldn’t find the skirt size unfortunately
Details can be found here
Skirt Height (mm) 6.096
Here additional dimensions, unfortunately they do not provide a drawing