Hello all. I have a Leica DMi8 TIRF microscope with a TIRF lens, 100x/1.47. The camera pixel size is 6.5um. I’d like to use it for dSTORM, and needs a 60x TIRF lens to reach ~100nm/pixel. Unfortunately, Leica has discontinued their 63x TIRF lens. I recently got an Olympus 60x/1.49 TIRF lens (APON60X…

I think that Leica uses a 200mm tube lens and Olympus uses 180mm. You didn’t mention if your calculation includes this, but if it doesn’t the pixel size might not be quite what you expect. However it should be close enough. Another option might be putting a de-magnifier in front of the camera and u…

Olympus and Nikon are generally interchangeable, just beware the 11% magnification mismatch Hazen noted. However Leica and Zeiss objective lenses are designed leaving some of the corrections to the tube lens, specifically lateral chromatic correction for both and possibly also curvature in the case…

Thank you @JonD for sharing the link. Can I say, other than the 10% magnification difference, Olympus objective can be used on Leica microscope. But the opposite is not true because the Leica objective relies on Leica tube lens to make more correction? Thank you @Hazen_Babcock for sharing. Ideally …

Yes that should work. The resolution of dSTORM is not particularly sensitive to pixel size.

Can I say, other than the 10% magnification difference, Olympus objective can be used on Leica microscope. But the opposite is not true because the Leica objective relies on Leica tube lens to make more correction? Neither the Leica tube lens and Leica objective and perfectly corrected alone but …

Thanks @JonD for clarifying. I will keep that in mind. Now I’m only interested in one-color. I will see how it goes with the M25-> RMS adapter, and compared it 100x with 0.7x C-mount.

Hi again @JonD . I was revisiting the reference you shared. amsikking: Microscope objectives Can you help me understand why the radius of the spherical cap is equal to [image] on the following diagram? [image] Regarding the Fourier space or the BFP, shouldn’t it be centered on the zero, and only …

Objective/microscope mix and match

Hardware

JonD February 25, 2025, 10:28pm 10

I didn’t immediately see which sub-page of Alfred’s repo that image is from so I can’t even read how Alfred labels the symbols. This is maybe a question better addressed to him.

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Objective/microscope mix and match

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