As you can see, I have a problem with black horizontal lines in my images that appear after I acquire it (they cannot be seen when looking through the microscope). I have tried unidirectional acquiring, decreasing the speed and nothing helped. I know some people with similar issues were experiencing problems with the table on which the microscope standa, but I doubt that is the problem for us? Any suggestions?
Thank you in advance:)
Are you seeing these lines only in the image display? Have you saved an image and tried to view it elsewhere?
We see this occasionally with certain large frame sizes and it is due to the graphics card not rendering the image display properly. The data is actually fine - but the display has a hard time keeping up with the huge data sets the Airyscan can render sometimes.
Thank you for answering. I will try to look at the image in ImageJ perhaps. So far, we only see black lines on PC display, not when looking directly through the microscope itself. I will update you. Have a nice weekend.
