Nikon Eclipse Ti back port to Back Focal Plane distance specification needed

Yossi_Steinberger
Fri, 15 Aug 2025 15:27:07 +0000
Hardware

Hello all,

I am designing a single-molecule microscope using a Nikon Eclipse Ti-E inverted microscope. My setup involves focusing a collimated laser beam onto the back focal plane of a 100×/1.4 NA objective via a ~200mm external lens mounted just outside the microscope’s back port (photo of the Thorlabs adapter attached).

Does anyone know the optical path distance from the microscope’s back port to the objective’s back focal plane? I haven’t found this specification in the product documentation.

Thank you,
Yossi

5 posts - 3 participants