What is the difference between Numerical Aperture and Effective Numerical Aperture?

Mauricio_Antonio
Thu, 09 Mar 2023 07:01:10 +0000
Hardware

Hi,
Going through a .czi metadata to extract some information I ran into both the NA 0.95 of the lens and a value set as the effective Numerical aperture a smaller value at 0.8. I wonder if anyone here can help me find this difference and from there to know which one theoretically is the one to be considered in the computatation of the point spread functio.

Many thanks!

2 posts - 2 participants