# \#super-resolution **URL:** https://forum.microlist.org/tag/super-resolution/16.md [Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md) --- ## [Achieving Uniform Widefield Illumination Without Vibrators](https://forum.microlist.org/t/achieving-uniform-widefield-illumination-without-vibrators/3324)
**Author:** [@Yossi\_Steinberger](https://forum.microlist.org/u/Yossi_Steinberger)\ **Replies:** 1\ **Last updated:** [April 13, 2026, 9:52pm UTC](https://forum.microlist.org/t/achieving-uniform-widefield-illumination-without-vibrators/3324 "2026-04-13T21:52:00Z")
Dear μforum community, I would like to add a 1W laser around 647nm in order to do single protein imaging. Specifically, I have a Nikon Ti2-E inverted widefield microscope, and want to achieve Kohler-based widefield illu… --- ## [Any anti-bleaching strategies for cells mounted with PBS?](https://forum.microlist.org/t/any-anti-bleaching-strategies-for-cells-mounted-with-pbs/3308)
**Author:** [@Topaz2026](https://forum.microlist.org/u/Topaz2026)\ **Replies:** 2\ **Last updated:** [February 17, 2026, 8:38am UTC](https://forum.microlist.org/t/any-anti-bleaching-strategies-for-cells-mounted-with-pbs/3308 "2026-02-17T08:38:52Z")
Hi everyone, I am now using water objective imaging samples in PBS, which provided no RI mismatch. But the sample in PBS was easier to bleach without any anti-bleaching agents added. I would like to change PBS to mounti… --- ## [Airyscan imaging issues](https://forum.microlist.org/t/airyscan-imaging-issues/2940)
**Author:** [@bufobufo](https://forum.microlist.org/u/bufobufo)\ **Replies:** 3\ **Last updated:** [December 31, 2024, 4:46am UTC](https://forum.microlist.org/t/airyscan-imaging-issues/2940 "2024-12-31T04:46:40Z")
Hi, I am a PhD student, and my project heavily depends on imaging. I’ve been using LSM880 for my confocal images; however, recently, my PI suggested I start using airyscan for some of my images. I am trying to visualize… --- ## [Xenium spatial resolution](https://forum.microlist.org/t/xenium-spatial-resolution/2929)
**Author:** [@smcardle](https://forum.microlist.org/u/smcardle)\ **Replies:** 5\ **Last updated:** [December 19, 2024, 6:41pm UTC](https://forum.microlist.org/t/xenium-spatial-resolution/2929 "2024-12-19T18:41:24Z")
10x Genomics claims that the Xenium provides sub-30nm resolution for single molecule RNA detection. I can’t find any data to back up their claim, or even any technical specs on their optics. Has anyone here had any exper… --- ## [Microscopy Conference AIM2025: Registration is Open!](https://forum.microlist.org/t/microscopy-conference-aim2025-registration-is-open/2873)
**Author:** [@cherise](https://forum.microlist.org/u/cherise)\ **Replies:** 0\ **Last updated:** [October 28, 2024, 8:16pm UTC](https://forum.microlist.org/t/microscopy-conference-aim2025-registration-is-open/2873 "2024-10-28T20:16:06Z")
Hello Microforum Community! We are gearing up for our 22nd annual Advanced Imaging Methods (AIM) Workshop here at UC Berkeley on Jan. 21st-23rd 2025. Registration is open: Early bird registration ends Nov. 1st 2024. I… --- ## [Basic microscopy Questions about building](https://forum.microlist.org/t/basic-microscopy-questions-about-building/2431)
**Author:** [@mediocremicroscoper](https://forum.microlist.org/u/mediocremicroscoper)\ **Replies:** 16\ **Last updated:** [April 26, 2024, 9:33am UTC](https://forum.microlist.org/t/basic-microscopy-questions-about-building/2431 "2024-04-26T09:33:27Z")
Hello everyone, Ive recently been tasked with building an inverted objective-tirf microscope. Im rather new to this so im learning by doing. I have built the system and aligned the laser, as far as I know. After alignin… --- ## [Search for AbberiorFlip565](https://forum.microlist.org/t/search-for-abberiorflip565/2337)
**Author:** [@HannahV](https://forum.microlist.org/u/HannahV)\ **Replies:** 0\ **Last updated:** [November 30, 2023, 8:57am UTC](https://forum.microlist.org/t/search-for-abberiorflip565/2337 "2023-11-30T08:57:50Z")
Hello, I’m searching for AbberiorFlip565 dye, but when I contacted Aberrior they told me that this series was discontinued. Would anyone know where I could potentially get it? Or would anyone be willing to share it/sel… --- ## [Guide to Microscopy about Cellular Ploidy Variation 2](https://forum.microlist.org/t/guide-to-microscopy-about-cellular-ploidy-variation-2/2307)
**Author:** [@Matheus\_C\_Casotti](https://forum.microlist.org/u/Matheus_C_Casotti)\ **Replies:** 0\ **Last updated:** [October 30, 2023, 1:46pm UTC](https://forum.microlist.org/t/guide-to-microscopy-about-cellular-ploidy-variation-2/2307 "2023-10-30T13:46:02Z")
Continuing the discussion from Guide to Microscopy about Cellular Ploidy Variation: Good morning My name is Matheus, I’m starting out in the area of image analysis and I would like to develop an analysis and identifica… --- ## [dSTORM camera and objective](https://forum.microlist.org/t/dstorm-camera-and-objective/2281)
**Author:** [@szh141](https://forum.microlist.org/u/szh141)\ **Replies:** 4\ **Last updated:** [September 22, 2023, 9:34pm UTC](https://forum.microlist.org/t/dstorm-camera-and-objective/2281 "2023-09-22T21:34:30Z")
Hello all. I have a Leica Thunder Imager with TIRF, 100x N.A. 1.47 and 4 laser lines with power between 100-250mW. I’m thinking to try dSTORM using the system. The camera on the system is Leica K8 sCMOS, 6.5um pixel, 95… --- ## [Camera for Lightsheet Microscopy](https://forum.microlist.org/t/camera-for-lightsheet-microscopy/1376)
**Author:** [@CARLOS\_CANTALLOPS\_IG](https://forum.microlist.org/u/CARLOS_CANTALLOPS_IG)\ **Replies:** 12\ **Last updated:** [May 25, 2022, 5:37am UTC](https://forum.microlist.org/t/camera-for-lightsheet-microscopy/1376 "2022-05-25T05:37:00Z")
Hi everyone, Im Carlos Cantallops a spanish student of UC3M. I would like to have some recommendations for the implementation of a camera on a new microscope I am working on. The microscopy is a Lighsheet Microscopy for… --- ## [Remote refocus High NA single-objective light-sheet,](https://forum.microlist.org/t/remote-refocus-high-na-single-objective-light-sheet/1637)
**Author:** [@Edalat](https://forum.microlist.org/u/Edalat)\ **Replies:** 2\ **Last updated:** [December 25, 2021, 10:59pm UTC](https://forum.microlist.org/t/remote-refocus-high-na-single-objective-light-sheet/1637 "2021-12-25T22:59:15Z")
Hi everybody, I am working on a remote focusing single objective light-sheet setup, following Andrew G. York; High NA single-objective light-sheet. Could anybody, who has some knowledge/experiences with such setup, ple… --- ## [Camera choices for STORM](https://forum.microlist.org/t/camera-choices-for-storm/1640)
**Author:** [@chao-wei](https://forum.microlist.org/u/chao-wei)\ **Replies:** 3\ **Last updated:** [December 19, 2021, 7:55pm UTC](https://forum.microlist.org/t/camera-choices-for-storm/1640 "2021-12-19T19:55:47Z")
Hi everyone, What is your suggestion on camera choices for a STORM system? sCMOS or EMCCD? I would imagine that a 6.5 μm sCMOS is better than a 16 μm EMCCD? But I was also told that if we are looking for localizations (… --- ## [Experience with Nanoimager and Vutara?](https://forum.microlist.org/t/experience-with-nanoimager-and-vutara/1513)
**Author:** [@chao-wei](https://forum.microlist.org/u/chao-wei)\ **Replies:** 0\ **Last updated:** [September 23, 2021, 3:54pm UTC](https://forum.microlist.org/t/experience-with-nanoimager-and-vutara/1513 "2021-09-23T15:54:10Z")
Hi, Does anyone have experience with Oni Nanoimager or Brucker Vutara VXL? We are in the process of buying one for the microscopy facility. Thank you! --- ## [AiryscanJ and raw airyscan image processing](https://forum.microlist.org/t/airyscanj-and-raw-airyscan-image-processing/1504)
**Author:** [@fadelvalle](https://forum.microlist.org/u/fadelvalle)\ **Replies:** 1\ **Last updated:** [September 18, 2021, 4:40pm UTC](https://forum.microlist.org/t/airyscanj-and-raw-airyscan-image-processing/1504 "2021-09-18T16:40:27Z")
Hi everyone! I was trying the AiryscanJ plugin since I have no place to process raw airyscan images for a long time and I constantly face the out of memory error when applying any of the methods. My pc has 32 GB of ram … --- ## [Why is my yellow dye signal very weak in dSTORM imaging?](https://forum.microlist.org/t/why-is-my-yellow-dye-signal-very-weak-in-dstorm-imaging/1391)
**Author:** [@Yunlong\_Zhao](https://forum.microlist.org/u/Yunlong_Zhao)\ **Replies:** 4\ **Last updated:** [June 7, 2021, 12:55pm UTC](https://forum.microlist.org/t/why-is-my-yellow-dye-signal-very-weak-in-dstorm-imaging/1391 "2021-06-07T12:55:59Z")
Dear all, I’m suffering from a very weak signal of yelow dye, such as CF568 and JF549, in my dSTORM imaging. I used yellow dye-conjugated Halo tag or antibody to stain my samples. Althrough I can see a very bright and s… --- ## [Nucleic acid stain-Chromatin banding-SIM](https://forum.microlist.org/t/nucleic-acid-stain-chromatin-banding-sim/1367)
**Author:** [@Chaowei](https://forum.microlist.org/u/Chaowei)\ **Replies:** 2\ **Last updated:** [May 31, 2021, 5:01pm UTC](https://forum.microlist.org/t/nucleic-acid-stain-chromatin-banding-sim/1367 "2021-05-31T17:01:45Z")
We are looking for a nucleic acid stain that can show chromatin banding, but does not fall into the DAPI and Hoechst wavelength. We need to observe chromatin banding on a SIM super resolution. However, this scope didn’t … --- ## [Experience using STEDYCON (STED) from Abberior?](https://forum.microlist.org/t/experience-using-stedycon-sted-from-abberior/1308)
**Author:** [@Chaowei](https://forum.microlist.org/u/Chaowei)\ **Replies:** 8\ **Last updated:** [March 25, 2021, 9:10pm UTC](https://forum.microlist.org/t/experience-using-stedycon-sted-from-abberior/1308 "2021-03-25T21:10:45Z")
Does anyone has experience with STEDYCON from Abberior? It is described as a user friendly and compact STED unit that can be added on to an existing microscope. What is your experience with it? Have you encountered any p… --- ## [Doubts on Airyscan](https://forum.microlist.org/t/doubts-on-airyscan/1231)
**Author:** [@lsilva.m](https://forum.microlist.org/u/lsilva.m)\ **Replies:** 1\ **Last updated:** [December 10, 2020, 11:46am UTC](https://forum.microlist.org/t/doubts-on-airyscan/1231 "2020-12-10T11:46:34Z")
\[this is a copy of a post originally posted in image.sc\] Hello, I am a PhD student, and recently begun to use the confocal microscope (LSM710) with Airyscan, and I have some doubts/curiosities—hopefully, you can help m… --- ## [SMLM set up question](https://forum.microlist.org/t/smlm-set-up-question/1193)
**Author:** [@snitkovs](https://forum.microlist.org/u/snitkovs)\ **Replies:** 14\ **Last updated:** [November 14, 2020, 1:31pm UTC](https://forum.microlist.org/t/smlm-set-up-question/1193 "2020-11-14T13:31:11Z")
We have a Nikon microscope. When I focus through 100 nm tetraspeck beads with an astigmatism lens in place, the shape of the bead is oval in the y-axis below the focal plane but it is not oval in the x-axis above the foc… --- ## [Advanced Microscopy & Data Analysis Position in Emory’s Integrated Cellular Imaging (ICI) Core](https://forum.microlist.org/t/advanced-microscopy-data-analysis-position-in-emory-s-integrated-cellular-imaging-ici-core/1168)
**Author:** [@nranthony](https://forum.microlist.org/u/nranthony)\ **Replies:** 0\ **Last updated:** [October 7, 2020, 8:22pm UTC](https://forum.microlist.org/t/advanced-microscopy-data-analysis-position-in-emory-s-integrated-cellular-imaging-ici-core/1168 "2020-10-07T20:22:22Z")
Hi all, I hope the science and scopes are treating you well. We have a position opening for an advanced microscopy and data analysis specialist in Emory’s Integrated Cellular Imaging (ICI) core facility. The role is cen… --- ## [The cell biologist's guide to super-resolution microscopy](https://forum.microlist.org/t/the-cell-biologists-guide-to-super-resolution-microscopy/1031)
**Author:** [@cleterrier](https://forum.microlist.org/u/cleterrier)\ **Replies:** 2\ **Last updated:** [September 8, 2020, 8:44pm UTC](https://forum.microlist.org/t/the-cell-biologists-guide-to-super-resolution-microscopy/1031 "2020-09-08T20:44:40Z")
Good morning, With Guillaume Jacquemet (U Turku), Alexandre Carisey (BCM Houston), Hellyeh Hamidi (U Turku) and Ricardo Henriques (@HenriquesLab UCL/Crick), we have just published in the “At A Glance” collection of J Ce… --- ## [Laser for single molecule super res imaging](https://forum.microlist.org/t/laser-for-single-molecule-super-res-imaging/882)
**Author:** [@Edalat](https://forum.microlist.org/u/Edalat)\ **Replies:** 11\ **Last updated:** [February 18, 2020, 4:42pm UTC](https://forum.microlist.org/t/laser-for-single-molecule-super-res-imaging/882 "2020-02-18T16:42:54Z")
Hello. Can anybody let me know what are the differences between a low quality laser and a high quality one, in terms of single molecule microscopy, with the same power (about 500 mW) and wavelength? I am talking about $2… --- ## [Photobleaching during live cell imaging experiment](https://forum.microlist.org/t/photobleaching-during-live-cell-imaging-experiment/759)
**Author:** [@Gaurav\_Joshi](https://forum.microlist.org/u/Gaurav_Joshi)\ **Replies:** 1\ **Last updated:** [January 7, 2020, 3:44pm UTC](https://forum.microlist.org/t/photobleaching-during-live-cell-imaging-experiment/759 "2020-01-07T15:44:11Z")
Hello, I am interested in doing some SIM live-cell imaging on OMX. One of the obvious problems that I am going to encounter is photobleaching of my sample. Apart from things that I can control (laser power, exposure and… --- ## [Maximum imaging depth for good STORM or PALM data?](https://forum.microlist.org/t/maximum-imaging-depth-for-good-storm-or-palm-data/90)
**Author:** [@Elizabeth](https://forum.microlist.org/u/Elizabeth)\ **Replies:** 5\ **Last updated:** [August 8, 2019, 10:02am UTC](https://forum.microlist.org/t/maximum-imaging-depth-for-good-storm-or-palm-data/90 "2019-08-08T10:02:37Z")
Realistically, how deep into tissue can you go while still achieving high-quality single molecule localization data? I’m sure this will vary based on the tissue, but just wondering what a reasonable ballpark would be. Th…