# \#sim
**URL:** https://forum.microlist.org/tag/sim/7.md
[Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md)
---
## [405-nm excitation conjugated phalloidin](https://forum.microlist.org/t/405-nm-excitation-conjugated-phalloidin/1788)
**Author:** [@cleterrier](https://forum.microlist.org/u/cleterrier)\
**Replies:** 1\
**Last updated:** [February 20, 2025, 11:10am UTC](https://forum.microlist.org/t/405-nm-excitation-conjugated-phalloidin/1788 "2025-02-20T11:10:50Z")
SIM/confocal folks, what’s the latest on usable fluorescent phalloidin with a 405 nm laser line? Our preferred option (DyLight 405) is not available. AF405+? Atto something? CF405M? iFluor 405? I don’t think Brilliant Vi…
---
## [How to generate PSF of iSIM using PSF generator](https://forum.microlist.org/t/how-to-generate-psf-of-isim-using-psf-generator/2381)
**Author:** [@Qiqi-Lu](https://forum.microlist.org/u/Qiqi-Lu)\
**Replies:** 6\
**Last updated:** [January 27, 2024, 8:34am UTC](https://forum.microlist.org/t/how-to-generate-psf-of-isim-using-psf-generator/2381 "2024-01-27T08:34:11Z")
Hello, I have troubles to generate a theorical PSF for instant SIM (iSIM) using PSF generator. For example, the iSIM mentioned in the paper ’ Guo, M. et al. Rapid image deconvolution and multiview fusion for optical mi…
---
## [Beads for channel alignment](https://forum.microlist.org/t/beads-for-channel-alignment/1197)
**Author:** [@sri1989vathsan](https://forum.microlist.org/u/sri1989vathsan)\
**Replies:** 2\
**Last updated:** [February 11, 2021, 7:53pm UTC](https://forum.microlist.org/t/beads-for-channel-alignment/1197 "2021-02-11T19:53:23Z")
I am looking for a set of 100nm beads that I can use for aligning different channels. I want to add these beads to my sample (as people do with EM) and image across the green (488 nm excitation), orange (561nm excitatio…