# \#nikon

**URL:** https://forum.microlist.org/tag/nikon/36.md

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## [Nikon Eclipse Ti back port to Back Focal Plane distance specification needed](https://forum.microlist.org/t/nikon-eclipse-ti-back-port-to-back-focal-plane-distance-specification-needed/3204)

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**Author:** [@Yossi\_Steinberger](https://forum.microlist.org/u/Yossi_Steinberger)\
**Replies:** 10\
**Last updated:** [August 17, 2026, 7:11am UTC](https://forum.microlist.org/t/nikon-eclipse-ti-back-port-to-back-focal-plane-distance-specification-needed/3204 "2026-08-17T07:11:48Z")

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Hello all, I am designing a single-molecule microscope using a Nikon Eclipse Ti-E inverted microscope. My setup involves focusing a collimated laser beam onto the back focal plane of a 100×/1.4 NA objective via a ~200mm…

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## [NIS Elements macro to replicate ND Sequence Acquisition](https://forum.microlist.org/t/nis-elements-macro-to-replicate-nd-sequence-acquisition/3274)

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**Author:** [@tswayne](https://forum.microlist.org/u/tswayne)\
**Replies:** 2\
**Last updated:** [November 19, 2025, 2:29pm UTC](https://forum.microlist.org/t/nis-elements-macro-to-replicate-nd-sequence-acquisition/3274 "2025-11-19T14:29:47Z")

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I would like to run a complicated XYZ/Lambda/PFS acquisition on a spinning-disk system with NIS Elements, recently upgraded to version 6. In Elements 5 and below, there was a great feature, “ND Sequence,” whereby you co…

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## [Drift in long-term multiposition imaging with Nikon PFS](https://forum.microlist.org/t/drift-in-long-term-multiposition-imaging-with-nikon-pfs/3117)

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**Author:** [@cleterrier](https://forum.microlist.org/u/cleterrier)\
**Replies:** 4\
**Last updated:** [May 23, 2025, 11:25am UTC](https://forum.microlist.org/t/drift-in-long-term-multiposition-imaging-with-nikon-pfs/3117 "2025-05-23T11:25:15Z")

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Hi, I’m writing here because I know there are good connoisseurs of the Nikon systems around here and hope to get some pointers from you! We are encountering an issue with Z-drift during long-term (\>24 hours) time-lapse…