# \#light-sheet **URL:** https://forum.microlist.org/tag/light-sheet/3.md [Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md) --- ## [Fringes on laser fiber output and impact on lightsheet quality](https://forum.microlist.org/t/fringes-on-laser-fiber-output-and-impact-on-lightsheet-quality/3412)
**Author:** [@Milo\_Imbeni](https://forum.microlist.org/u/Milo_Imbeni)\ **Replies:** 4\ **Last updated:** [October 1, 2026, 3:48pm UTC](https://forum.microlist.org/t/fringes-on-laser-fiber-output-and-impact-on-lightsheet-quality/3412 "2026-10-01T15:48:44Z")
Hi everyone! I work with a dual view lightsheet microscope, with the laser launched from an NKT select with two outputs. We then input the fiber in an ASI scanner. One side has consistently performed worse in terms of sh… --- ## [Micronaut: a browser-based planner for microscopy experiments](https://forum.microlist.org/t/micronaut-a-browser-based-planner-for-microscopy-experiments/3409)
**Author:** [@Daniel-Waiger](https://forum.microlist.org/u/Daniel-Waiger)\ **Replies:** 0\ **Last updated:** [September 9, 2026, 11:00am UTC](https://forum.microlist.org/t/micronaut-a-browser-based-planner-for-microscopy-experiments/3409 "2026-09-09T11:00:03Z")
Hi all, I have been building a small free tool called Micronaut and would value this community’s eyes on it. It is a planner for the stage before acquisition: the point where you decide what you are comparing, what coun… --- ## [Microscope camera software](https://forum.microlist.org/t/microscope-camera-software/3250)
**Author:** [@Fried](https://forum.microlist.org/u/Fried)\ **Replies:** 2\ **Last updated:** [October 7, 2025, 11:50am UTC](https://forum.microlist.org/t/microscope-camera-software/3250 "2025-10-07T11:50:13Z")
Can anyone provide me with Swift Easy View software --- ## [What are the main causes of poor axial resolution in light sheet microscopy? (SPIM)](https://forum.microlist.org/t/what-are-the-main-causes-of-poor-axial-resolution-in-light-sheet-microscopy-spim/3151)
**Author:** [@engpol](https://forum.microlist.org/u/engpol)\ **Replies:** 5\ **Last updated:** [July 10, 2025, 12:29pm UTC](https://forum.microlist.org/t/what-are-the-main-causes-of-poor-axial-resolution-in-light-sheet-microscopy-spim/3151 "2025-07-10T12:29:52Z")
First post on here, apologies beforehand for any mistakes in etiquette. I should also say that I am very new to the field of light sheet microscopy, so please do correct me if anything I say is misguided. P.S Apparently… --- ## [Any experience with solvent resistant materials for sample holding in lightsheet](https://forum.microlist.org/t/any-experience-with-solvent-resistant-materials-for-sample-holding-in-lightsheet/89)
**Author:** [@GabyS](https://forum.microlist.org/u/GabyS)\ **Replies:** 7\ **Last updated:** [June 13, 2025, 9:59am UTC](https://forum.microlist.org/t/any-experience-with-solvent-resistant-materials-for-sample-holding-in-lightsheet/89 "2025-06-13T09:59:37Z")
We have the Ultra-microscope II in our facility and would like to 3D print some custom holders. Anyone has experience with good materials to use? We are using for most cases Ethyl cinnamate as imaging media. Thanks --- ## [Guide to Microscopy about Cellular Ploidy Variation](https://forum.microlist.org/t/guide-to-microscopy-about-cellular-ploidy-variation/2301)
**Author:** [@Matheus\_C\_Casotti](https://forum.microlist.org/u/Matheus_C_Casotti)\ **Replies:** 2\ **Last updated:** [February 13, 2025, 8:54pm UTC](https://forum.microlist.org/t/guide-to-microscopy-about-cellular-ploidy-variation/2301 "2025-02-13T20:54:47Z")
Good morning My name is Matheus, I’m starting out in the area of image analysis and I would like to develop an analysis and identification of polyploid cells in histological slides stained in HE and also with fluorescen… --- ## [Zeiss Lightsheet 7 filter cubes?](https://forum.microlist.org/t/zeiss-lightsheet-7-filter-cubes/2928)
**Author:** [@michael](https://forum.microlist.org/u/michael)\ **Replies:** 3\ **Last updated:** [December 18, 2024, 3:55pm UTC](https://forum.microlist.org/t/zeiss-lightsheet-7-filter-cubes/2928 "2024-12-18T15:55:04Z")
Hello everyone, I’m looking for empty filter cubes for the detection path of the Zeiss Lightsheet 7 (former Z.1) to test emission filters. Unfortunately, Zeiss is having a hard time providing these. So I was wondering if… --- ## [Why isn't there a competition or benchmarking for microscopes?](https://forum.microlist.org/t/why-isnt-there-a-competition-or-benchmarking-for-microscopes/2828)
**Author:** [@macromeer](https://forum.microlist.org/u/macromeer)\ **Replies:** 7\ **Last updated:** [October 17, 2024, 9:05am UTC](https://forum.microlist.org/t/why-isnt-there-a-competition-or-benchmarking-for-microscopes/2828 "2024-10-17T09:05:08Z")
An honest question. I am currently comparing spinning disc to light sheet and I am wondering why it is practically impossible to find image data of the same specimen from both light sheet and spinning disc. Is it imposs… --- ## [Guide to Microscopy about Cellular Ploidy Variation 2](https://forum.microlist.org/t/guide-to-microscopy-about-cellular-ploidy-variation-2/2307)
**Author:** [@Matheus\_C\_Casotti](https://forum.microlist.org/u/Matheus_C_Casotti)\ **Replies:** 0\ **Last updated:** [October 30, 2023, 1:46pm UTC](https://forum.microlist.org/t/guide-to-microscopy-about-cellular-ploidy-variation-2/2307 "2023-10-30T13:46:02Z")
Continuing the discussion from Guide to Microscopy about Cellular Ploidy Variation: Good morning My name is Matheus, I’m starting out in the area of image analysis and I would like to develop an analysis and identifica… --- ## [Is it possible to re-slice cleared samples?](https://forum.microlist.org/t/is-it-possible-to-re-slice-cleared-samples/2217)
**Author:** [@J\_Ramirez](https://forum.microlist.org/u/J_Ramirez)\ **Replies:** 4\ **Last updated:** [August 30, 2023, 10:03am UTC](https://forum.microlist.org/t/is-it-possible-to-re-slice-cleared-samples/2217 "2023-08-30T10:03:21Z")
Hi there everyone, I was thinking about re-slicing samples that have undergone clearing through the DISCO method (vDISCO) in order to image them in a confocal system. Ideally, we would like to register the confocal aqui… --- ## [Alternattive to tetraspeck beads in lightsheet](https://forum.microlist.org/t/alternattive-to-tetraspeck-beads-in-lightsheet/2232)
**Author:** [@jbogomolovas2](https://forum.microlist.org/u/jbogomolovas2)\ **Replies:** 1\ **Last updated:** [August 4, 2023, 2:34pm UTC](https://forum.microlist.org/t/alternattive-to-tetraspeck-beads-in-lightsheet/2232 "2023-08-04T14:34:28Z")
LHello Everyone, I am currently using fluorescent tetraspeck beads for image registration in lightsheet multiangle microscopy. However, I’ve noticed that these beads display a notably weak signal in the DAPI channel, w… --- ## [Blurring of light sheet](https://forum.microlist.org/t/blurring-of-light-sheet/1892)
**Author:** [@GC\_PENG](https://forum.microlist.org/u/GC_PENG)\ **Replies:** 3\ **Last updated:** [September 13, 2022, 2:34pm UTC](https://forum.microlist.org/t/blurring-of-light-sheet/1892 "2022-09-13T14:34:10Z")
Dear experts, I am a student from China. Recently, when I used the light sheet7 (Zeiss) light microscope to image the pancreatic islets, there was blurring. However, it was very clear during the two-dimensional scanning.… --- ## [Image splitting](https://forum.microlist.org/t/image-splitting/1914)
**Author:** [@micermi](https://forum.microlist.org/u/micermi)\ **Replies:** 0\ **Last updated:** [September 13, 2022, 9:44am UTC](https://forum.microlist.org/t/image-splitting/1914 "2022-09-13T09:44:41Z")
Hello, can anyone help me choosing between the Hamamatsu W-view Gemini and the CairnResearch Optosplit to achieve simultaneous two-colours imaging by splitting the image on the same camera chip? Thanks in advance, Mic… --- ## [Camera for Lightsheet Microscopy](https://forum.microlist.org/t/camera-for-lightsheet-microscopy/1376)
**Author:** [@CARLOS\_CANTALLOPS\_IG](https://forum.microlist.org/u/CARLOS_CANTALLOPS_IG)\ **Replies:** 12\ **Last updated:** [May 25, 2022, 5:37am UTC](https://forum.microlist.org/t/camera-for-lightsheet-microscopy/1376 "2022-05-25T05:37:00Z")
Hi everyone, Im Carlos Cantallops a spanish student of UC3M. I would like to have some recommendations for the implementation of a camera on a new microscope I am working on. The microscopy is a Lighsheet Microscopy for… --- ## [Bacterial staining on formaline fixed spheroids](https://forum.microlist.org/t/bacterial-staining-on-formaline-fixed-spheroids/1697)
**Author:** [@pootaj](https://forum.microlist.org/u/pootaj)\ **Replies:** 0\ **Last updated:** [January 24, 2022, 4:09pm UTC](https://forum.microlist.org/t/bacterial-staining-on-formaline-fixed-spheroids/1697 "2022-01-24T16:09:47Z")
Hi, I am working with human cells spheroids model. I am infecting spheroids with bacteria and using Baclight red bacterial stain for visualising bacteria in the spheroid. I have noticed that Baclight red stains bacteria… --- ## [Remote refocus High NA single-objective light-sheet,](https://forum.microlist.org/t/remote-refocus-high-na-single-objective-light-sheet/1637)
**Author:** [@Edalat](https://forum.microlist.org/u/Edalat)\ **Replies:** 2\ **Last updated:** [December 25, 2021, 10:59pm UTC](https://forum.microlist.org/t/remote-refocus-high-na-single-objective-light-sheet/1637 "2021-12-25T22:59:15Z")
Hi everybody, I am working on a remote focusing single objective light-sheet setup, following Andrew G. York; High NA single-objective light-sheet. Could anybody, who has some knowledge/experiences with such setup, ple… --- ## [Transfer from DBE to ECi protocol](https://forum.microlist.org/t/transfer-from-dbe-to-eci-protocol/1597)
**Author:** [@jana](https://forum.microlist.org/u/jana)\ **Replies:** 0\ **Last updated:** [November 4, 2021, 10:19am UTC](https://forum.microlist.org/t/transfer-from-dbe-to-eci-protocol/1597 "2021-11-04T10:19:28Z")
Hello, I am planing to transfer the brain samples stored in DBE to ECi for imaging. Does anyone could help with the protocol how to do it? Thank you --- ## [Zeiss lattice lightsheet experience](https://forum.microlist.org/t/zeiss-lattice-lightsheet-experience/1437)
**Author:** [@lisacam7](https://forum.microlist.org/u/lisacam7)\ **Replies:** 1\ **Last updated:** [June 18, 2021, 1:03pm UTC](https://forum.microlist.org/t/zeiss-lattice-lightsheet-experience/1437 "2021-06-18T13:03:54Z")
Hi all - I’m wondering if people have demo-ed, ordered, installed or tried the Zeiss lattice lightsheet system. How did it work for you? pluses? minuses? Feel free to contact me privately if preferred lisa.cameron@duke.e… --- ## [PSF of SPIM systems and deconvolution](https://forum.microlist.org/t/psf-of-spim-systems-and-deconvolution/1346)
**Author:** [@manerotoni](https://forum.microlist.org/u/manerotoni)\ **Replies:** 13\ **Last updated:** [April 26, 2021, 7:58am UTC](https://forum.microlist.org/t/psf-of-spim-systems-and-deconvolution/1346 "2021-04-26T07:58:18Z")
Hello, I was wondering what is your experience for deconvolution of ligh-sheet data. in particular what is the best way to compute/estimate the PSF. For a classic light-sheet with gaussian beam the light-sheet thickne… --- ## [Advanced Microscopy & Data Analysis Position in Emory’s Integrated Cellular Imaging (ICI) Core](https://forum.microlist.org/t/advanced-microscopy-data-analysis-position-in-emory-s-integrated-cellular-imaging-ici-core/1168)
**Author:** [@nranthony](https://forum.microlist.org/u/nranthony)\ **Replies:** 0\ **Last updated:** [October 7, 2020, 8:22pm UTC](https://forum.microlist.org/t/advanced-microscopy-data-analysis-position-in-emory-s-integrated-cellular-imaging-ici-core/1168 "2020-10-07T20:22:22Z")
Hi all, I hope the science and scopes are treating you well. We have a position opening for an advanced microscopy and data analysis specialist in Emory’s Integrated Cellular Imaging (ICI) core facility. The role is cen… --- ## [Light Sheet Microscopy Helpline](https://forum.microlist.org/t/light-sheet-microscopy-helpline/1155)
**Author:** [@Emmanuel\_Reynaud](https://forum.microlist.org/u/Emmanuel_Reynaud)\ **Replies:** 0\ **Last updated:** [September 28, 2020, 9:44pm UTC](https://forum.microlist.org/t/light-sheet-microscopy-helpline/1155 "2020-09-28T21:44:47Z")
Dear Microforum, Light Sheet Fluorescence Microscopy is now an optical imaging method found across the world. However it is quite a different illumination method that comes with a few issues for users. We have started a… --- ## [Laser for single molecule super res imaging](https://forum.microlist.org/t/laser-for-single-molecule-super-res-imaging/882)
**Author:** [@Edalat](https://forum.microlist.org/u/Edalat)\ **Replies:** 11\ **Last updated:** [February 18, 2020, 4:42pm UTC](https://forum.microlist.org/t/laser-for-single-molecule-super-res-imaging/882 "2020-02-18T16:42:54Z")
Hello. Can anybody let me know what are the differences between a low quality laser and a high quality one, in terms of single molecule microscopy, with the same power (about 500 mW) and wavelength? I am talking about $2… --- ## [DNA labelling for lightsheet microscope](https://forum.microlist.org/t/dna-labelling-for-lightsheet-microscope/718)
**Author:** [@Alex.h](https://forum.microlist.org/u/Alex.h)\ **Replies:** 3\ **Last updated:** [January 28, 2020, 3:47am UTC](https://forum.microlist.org/t/dna-labelling-for-lightsheet-microscope/718 "2020-01-28T03:47:13Z")
Dear all, We use different protocol to clear our organ like X-clarity, CUBIC, RapiClear etc… But we have some problem with the DNA Staining. I measure that we stained with DAPI +/- 600 µm. The majority of our samples ar… --- ## [Mineral and silicone oil for Lightsheet microscopy](https://forum.microlist.org/t/mineral-and-silicone-oil-for-lightsheet-microscopy/822)
**Author:** [@Alex.h](https://forum.microlist.org/u/Alex.h)\ **Replies:** 2\ **Last updated:** [January 27, 2020, 11:00am UTC](https://forum.microlist.org/t/mineral-and-silicone-oil-for-lightsheet-microscopy/822 "2020-01-27T11:00:51Z")
Dear all, We are looking to use oil for refractive index matching with the new version of the CUBIC (CUBIC-L and CUBIC -R). We need large quantities (30ml of mililiters for each run). The final RI will be 1.52 Does an… --- ## [Non-Fluorescent Silicon Glue](https://forum.microlist.org/t/non-fluorescent-silicon-glue/750)
**Author:** [@Gideon\_Oluniran](https://forum.microlist.org/u/Gideon_Oluniran)\ **Replies:** 0\ **Last updated:** [December 5, 2019, 12:11pm UTC](https://forum.microlist.org/t/non-fluorescent-silicon-glue/750 "2019-12-05T12:11:28Z")
Good day, Can someone please suggest a non-fluorescent silicon glue? I’ll be using them for imaging on a light sheet microscope (488nm excitation mostlyt) but do not want them interfering with my fluorescent images. Gi… --- ## [Clearing protocole for Lightsheet microscopy](https://forum.microlist.org/t/clearing-protocole-for-lightsheet-microscopy/512)
**Author:** [@Alex.h](https://forum.microlist.org/u/Alex.h)\ **Replies:** 6\ **Last updated:** [September 12, 2019, 8:01am UTC](https://forum.microlist.org/t/clearing-protocole-for-lightsheet-microscopy/512 "2019-09-12T08:01:18Z")
Hello, I read some article, and I found a lot of different protocol for the clearing. We already use some protocol in our platform with a lightsheet Z1 from Zeiss. X clarity for adult mice brain CUBIC for organoid an…