# \#imagej

**URL:** https://forum.microlist.org/tag/imagej/23.md

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## [Upcoming I2K meetings: Online (2020) & In-Person (2021)](https://forum.microlist.org/t/upcoming-i2k-meetings-online-2020-in-person-2021/1038)

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**Author:** [@etadobson](https://forum.microlist.org/u/etadobson)\
**Replies:** 0\
**Last updated:** [June 16, 2020, 6:50pm UTC](https://forum.microlist.org/t/upcoming-i2k-meetings-online-2020-in-person-2021/1038 "2020-06-16T18:50:23Z")

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Dear Imaging community, As many of you know, “From Images to Knowledge with ImageJ & Friends (I2K)” was scheduled for September 17-18, 2020 in Madison, WI. Due to travel restrictions during this continued health crisis …

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## [Slicing tissue blocks](https://forum.microlist.org/t/slicing-tissue-blocks/870)

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**Author:** [@Remi\_A](https://forum.microlist.org/u/Remi_A)\
**Replies:** 2\
**Last updated:** [February 14, 2020, 2:25pm UTC](https://forum.microlist.org/t/slicing-tissue-blocks/870 "2020-02-14T14:25:53Z")

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I will appreciate if anyone could tip me about how best to position and make thin slices (4-5um) from tissue blocks when such tissue blocks are irregular in shape, without wasting these samples. For context, these blocks…

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## [Cell counting with ImageJ](https://forum.microlist.org/t/cell-counting-with-imagej/817)

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**Author:** [@Katie\_Lui](https://forum.microlist.org/u/Katie_Lui)\
**Replies:** 2\
**Last updated:** [January 21, 2020, 2:21pm UTC](https://forum.microlist.org/t/cell-counting-with-imagej/817 "2020-01-21T14:21:39Z")

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I was trying to do cell counting analysis on some of my cultured rat astrocytes whose boundaries are quite indistinct as the cells are flat and confluent, by watershed after having done some adjustment to the images. The…

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## [Area changes with Threshold change in ImageJ?](https://forum.microlist.org/t/area-changes-with-threshold-change-in-imagej/785)

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**Author:** [@dschnitzler](https://forum.microlist.org/u/dschnitzler)\
**Replies:** 5\
**Last updated:** [January 14, 2020, 10:18pm UTC](https://forum.microlist.org/t/area-changes-with-threshold-change-in-imagej/785 "2020-01-14T22:18:42Z")

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Hi All Just signed up to ask about this: I select my ROI in ImageJ and measure and I get a number for the area (say 114611.775). I then adjust the threshold to cover only the particles I’m interested in measuring, measu…

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## [ImageJ macro control of Micro-Manager2](https://forum.microlist.org/t/imagej-macro-control-of-micro-manager2/487)

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**Author:** [@mutterer](https://forum.microlist.org/u/mutterer)\
**Replies:** 1\
**Last updated:** [September 3, 2019, 5:04pm UTC](https://forum.microlist.org/t/imagej-macro-control-of-micro-manager2/487 "2019-09-03T17:04:52Z")

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If you use Micro-Manager2, I would like to let you know that I wrote a set of extensions to perform basic microscope control from your ImageJ macros. Examples and source code is available at https://github.com/mutterer/…