# \#confocal **URL:** https://forum.microlist.org/tag/confocal/25.md [Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md) --- ## [How to display/setup phase contrast/brighfield images into the camera in a Nikon Ti microscope](https://forum.microlist.org/t/how-to-display-setup-phase-contrast-brighfield-images-into-the-camera-in-a-nikon-ti-microscope/3424)
**Author:** [@microtcell](https://forum.microlist.org/u/microtcell)\ **Replies:** 1\ **Last updated:** [November 4, 2026, 6:15pm UTC](https://forum.microlist.org/t/how-to-display-setup-phase-contrast-brighfield-images-into-the-camera-in-a-nikon-ti-microscope/3424 "2026-11-04T18:15:18Z")
Hi everyone, Can someone walk me through the steps or share a manual on how to setup phase contrast images and get them to the camera in a Nikon Ti microscope? I do have appropriate objectives and also a phse contrast r… --- ## [Help and guidance on installing EM filter on CSU-W1 Yokogawa confocal head](https://forum.microlist.org/t/help-and-guidance-on-installing-em-filter-on-csu-w1-yokogawa-confocal-head/3421)
**Author:** [@microtcell](https://forum.microlist.org/u/microtcell)\ **Replies:** 2\ **Last updated:** [October 10, 2026, 10:04pm UTC](https://forum.microlist.org/t/help-and-guidance-on-installing-em-filter-on-csu-w1-yokogawa-confocal-head/3421 "2026-10-10T22:04:55Z")
Hello everyone, We got a new EM filter we would like to install in our filter wheel on our Yokogawa CSU-W1 confocal unit. I was wondering if you have any tips to do so, and also remove potential dust particles that are … --- ## [vDISCO Troubleshooting](https://forum.microlist.org/t/vdisco-troubleshooting/3414)
**Author:** [@pineapple](https://forum.microlist.org/u/pineapple)\ **Replies:** 1\ **Last updated:** [October 3, 2026, 7:31am UTC](https://forum.microlist.org/t/vdisco-troubleshooting/3414 "2026-10-03T07:31:25Z")
Hi! I am trying to reproduce the vDISCO protocol and have been running into some issues when imaging with the lightsheet. In the future, I hope to use this protocol to image fine nerve fibers in the face so having a qu… --- ## [Switching board for bypassing Confocal.nl devices](https://forum.microlist.org/t/switching-board-for-bypassing-confocal-nl-devices/3416)
**Author:** [@Omnistic](https://forum.microlist.org/u/Omnistic)\ **Replies:** 0\ **Last updated:** [October 2, 2026, 2:08pm UTC](https://forum.microlist.org/t/switching-board-for-bypassing-confocal-nl-devices/3416 "2026-10-02T14:08:56Z")
Hi all, In our facility, we’ve had to deal with a specific issue of Confocal.nl devices. Those devices have a controller that directly modulates the lasers and triggers the camera. The device has a bypass mode, meaning … --- ## [Micronaut: a browser-based planner for microscopy experiments](https://forum.microlist.org/t/micronaut-a-browser-based-planner-for-microscopy-experiments/3409)
**Author:** [@Daniel-Waiger](https://forum.microlist.org/u/Daniel-Waiger)\ **Replies:** 0\ **Last updated:** [September 9, 2026, 11:00am UTC](https://forum.microlist.org/t/micronaut-a-browser-based-planner-for-microscopy-experiments/3409 "2026-09-09T11:00:03Z")
Hi all, I have been building a small free tool called Micronaut and would value this community’s eyes on it. It is a planner for the stage before acquisition: the point where you decide what you are comparing, what coun… --- ## [Digital Offset settings Confocal PMT](https://forum.microlist.org/t/digital-offset-settings-confocal-pmt/1372)
**Author:** [@Mitchell\_Han](https://forum.microlist.org/u/Mitchell_Han)\ **Replies:** 9\ **Last updated:** [August 25, 2026, 11:57am UTC](https://forum.microlist.org/t/digital-offset-settings-confocal-pmt/1372 "2026-08-25T11:57:42Z")
Dear all, I recently watched the excellent training video for the LSM880 by Jason Kirk for reference of our own facility. In his video he mentions using the Digital Offset slider for the PMT to eliminate “blue” (underex… --- ## [Problem with Zeiss LSM 800 + Airyscan: No Confocal Communication in ZEN 3.3](https://forum.microlist.org/t/problem-with-zeiss-lsm-800-airyscan-no-confocal-communication-in-zen-3-3/3203)
**Author:** [@saltfishpoi](https://forum.microlist.org/u/saltfishpoi)\ **Replies:** 4\ **Last updated:** [August 6, 2026, 7:32pm UTC](https://forum.microlist.org/t/problem-with-zeiss-lsm-800-airyscan-no-confocal-communication-in-zen-3-3/3203 "2026-08-06T19:32:21Z")
Hello everyone, Our lab was recently reorganized, and we have set up a Zeiss LSM 800 with an Airyscan detector. After some effort, we located the original control computer that was in storage and are now facing a commun… --- ## [LSM 780 Spectral Detector Servo Motor Map](https://forum.microlist.org/t/lsm-780-spectral-detector-servo-motor-map/3394)
**Author:** [@llamero](https://forum.microlist.org/u/llamero)\ **Replies:** 1\ **Last updated:** [August 3, 2026, 2:35pm UTC](https://forum.microlist.org/t/lsm-780-spectral-detector-servo-motor-map/3394 "2026-08-03T14:35:11Z")
Hey Microscopists, In case this is helpful to anyone else, I went ahead and mapped out the motors in the spectral detector for the LSM 780: LSM 780 Spectral Detector Motor Map.pptx - Google Slides Cheers, Ben Smith --- ## [Looking for relicts of Leica TCS-SP2](https://forum.microlist.org/t/looking-for-relicts-of-leica-tcs-sp2/2422)
**Author:** [@blaubaer](https://forum.microlist.org/u/blaubaer)\ **Replies:** 5\ **Last updated:** [July 17, 2026, 10:32am UTC](https://forum.microlist.org/t/looking-for-relicts-of-leica-tcs-sp2/2422 "2026-07-17T10:32:27Z")
25 year old Leica TCS SP2 is far out of professional awareness but it may be still charming for hobby use. I have collected the AOTF-scanhead, supply station plus other hardware complete and the right LCS version as well… --- ## [Optimizing for large-area imaging](https://forum.microlist.org/t/optimizing-for-large-area-imaging/3303)
**Author:** [@Amirali](https://forum.microlist.org/u/Amirali)\ **Replies:** 1\ **Last updated:** [March 2, 2026, 9:47am UTC](https://forum.microlist.org/t/optimizing-for-large-area-imaging/3303 "2026-03-02T09:47:05Z")
Hi everyone, I am working on a CyCIF project and need to image a large tissue area (approx. 2cm x 2cm). I am using an Olympus FV3000 point-scanning confocal. In previous pilot runs on smaller areas, I used a 20x (NA 0.… --- ## [Black Stripes on Custom made Laser Scan Confocal Miscroscope](https://forum.microlist.org/t/black-stripes-on-custom-made-laser-scan-confocal-miscroscope/2573)
**Author:** [@Augusto\_Vieira](https://forum.microlist.org/u/Augusto_Vieira)\ **Replies:** 7\ **Last updated:** [February 19, 2026, 1:54pm UTC](https://forum.microlist.org/t/black-stripes-on-custom-made-laser-scan-confocal-miscroscope/2573 "2026-02-19T13:54:15Z")
Greetings. I am currently building an laser scan confocal microscope to be used in my Master’s project. Optical elements such as galvo scan mirrors, lenses, laser, filters and PMT are assembled on a breadboard and the s… --- ## [Any anti-bleaching strategies for cells mounted with PBS?](https://forum.microlist.org/t/any-anti-bleaching-strategies-for-cells-mounted-with-pbs/3308)
**Author:** [@Topaz2026](https://forum.microlist.org/u/Topaz2026)\ **Replies:** 2\ **Last updated:** [February 17, 2026, 8:38am UTC](https://forum.microlist.org/t/any-anti-bleaching-strategies-for-cells-mounted-with-pbs/3308 "2026-02-17T08:38:52Z")
Hi everyone, I am now using water objective imaging samples in PBS, which provided no RI mismatch. But the sample in PBS was easier to bleach without any anti-bleaching agents added. I would like to change PBS to mounti… --- ## [Yokogawa CSUX1 Shutter 'sticking'? Lifetime surpassed?](https://forum.microlist.org/t/yokogawa-csux1-shutter-sticking-lifetime-surpassed/3248)
**Author:** [@CMCI](https://forum.microlist.org/u/CMCI)\ **Replies:** 4\ **Last updated:** [October 3, 2025, 3:08pm UTC](https://forum.microlist.org/t/yokogawa-csux1-shutter-sticking-lifetime-surpassed/3248 "2025-10-03T15:08:03Z")
We are having an issue with our CSUX1-based system in which the CSUX1 shutter is “sticking”; specifically, it’s not opening 100% of the time that it should be. When capturing a multipoint image or a multi-timepoint image… --- ## [LSM 7MP scanner calibration fails](https://forum.microlist.org/t/lsm-7mp-scanner-calibration-fails/3249)
**Author:** [@chsm](https://forum.microlist.org/u/chsm)\ **Replies:** 0\ **Last updated:** [October 2, 2025, 7:39pm UTC](https://forum.microlist.org/t/lsm-7mp-scanner-calibration-fails/3249 "2025-10-02T19:39:17Z")
Hi Folks, I reinstalled an old Zeiss LSM 7MP system and aligned the NLO laser. Scanner calibration/scan field test keep failing and I noticed the adjustment laser within the scan head used for these tests may not be ali… --- ## [Olympus FV1000 Laser Emission Problem](https://forum.microlist.org/t/olympus-fv1000-laser-emission-problem/3210)
**Author:** [@jfimbery](https://forum.microlist.org/u/jfimbery)\ **Replies:** 6\ **Last updated:** [September 12, 2025, 8:00am UTC](https://forum.microlist.org/t/olympus-fv1000-laser-emission-problem/3210 "2025-09-12T08:00:53Z")
Hi, I am using an Olympus FV1000 confocal microscope. Usually when imaging, there are 2 lights which illuminate and indicate ‘laser emission’ is on. However, I could not get them to work and thus no imaging could occur.… --- ## [Service Provider for Olympus FV1000 Confocal - Scandinavia](https://forum.microlist.org/t/service-provider-for-olympus-fv1000-confocal-scandinavia/3231)
**Author:** [@jfimbery](https://forum.microlist.org/u/jfimbery)\ **Replies:** 0\ **Last updated:** [September 12, 2025, 7:57am UTC](https://forum.microlist.org/t/service-provider-for-olympus-fv1000-confocal-scandinavia/3231 "2025-09-12T07:57:29Z")
Hi, our research group is located in Oslo, Norway. We may need some repair work on an Olympus FV1000 confocal microscope. Anyone have a local suggestion? Naturally, we are hoping to obtain a quote for what the repair wor… --- ## [Olympus Extended focal imaging (EFI) algorithm](https://forum.microlist.org/t/olympus-extended-focal-imaging-efi-algorithm/3212)
**Author:** [@isv](https://forum.microlist.org/u/isv)\ **Replies:** 3\ **Last updated:** [September 5, 2025, 7:48am UTC](https://forum.microlist.org/t/olympus-extended-focal-imaging-efi-algorithm/3212 "2025-09-05T07:48:22Z")
Dear all, Can anybody explain the algorithm behind the Extended Focal Imaging option available in Olympus CellSens? I understand that it is some form of z-stack projection that is performed before the image is saved, bu… --- ## [Hacking and old Leica TCS SP2](https://forum.microlist.org/t/hacking-and-old-leica-tcs-sp2/2077)
**Author:** [@rcphysics](https://forum.microlist.org/u/rcphysics)\ **Replies:** 2\ **Last updated:** [June 9, 2025, 8:48pm UTC](https://forum.microlist.org/t/hacking-and-old-leica-tcs-sp2/2077 "2025-06-09T20:48:39Z")
I received an old Leica setup that our medical school was getting rid of. IT consists of a TCS SP2 scan unit with PMTs and galvo scanners, some lasers, and a DMIRBE. Is there anyone here who has advice for salvaging the… --- ## [OlympusFV1000 Operational Help - Error Message - Confocal Microscopy](https://forum.microlist.org/t/olympusfv1000-operational-help-error-message-confocal-microscopy/3072)
**Author:** [@jfimbery](https://forum.microlist.org/u/jfimbery)\ **Replies:** 4\ **Last updated:** [May 14, 2025, 12:17pm UTC](https://forum.microlist.org/t/olympusfv1000-operational-help-error-message-confocal-microscopy/3072 "2025-05-14T12:17:55Z")
Hi, our research group is using an older Olympus FV1000 confocal microscope and an BX-UCB Olympus control box. Today, an error message appeared stating ‘failed in changing light path. please confirm the state of H/W’. Th… --- ## [Strange pixel / dots artifact in red channel of laser scanning confocal](https://forum.microlist.org/t/strange-pixel-dots-artifact-in-red-channel-of-laser-scanning-confocal/3062)
**Author:** [@AnKo](https://forum.microlist.org/u/AnKo)\ **Replies:** 2\ **Last updated:** [April 16, 2025, 5:41am UTC](https://forum.microlist.org/t/strange-pixel-dots-artifact-in-red-channel-of-laser-scanning-confocal/3062 "2025-04-16T05:41:31Z")
Dear all, we have a Nikon A1R laser scanning confocal microscope in our lab, and recently I’ve been seeing a strange “overexposed pixel” issue in the red channel only. Bright comet-like dots the size of one pixel appear… --- ## [Image Saturation - No blue pixels vs "few" blue pixels](https://forum.microlist.org/t/image-saturation-no-blue-pixels-vs-few-blue-pixels/1296)
**Author:** [@phoTONIcs](https://forum.microlist.org/u/phoTONIcs)\ **Replies:** 12\ **Last updated:** [March 16, 2025, 1:52pm UTC](https://forum.microlist.org/t/image-saturation-no-blue-pixels-vs-few-blue-pixels/1296 "2025-03-16T13:52:23Z")
A majority of core facilities have differing protocols when it comes to setting confocal parameters. According to Leica, you want a few green and a few blue pixels when looking at the LUT. Other protocols/SOPs state you … --- ## [How does PMT creates pixels?](https://forum.microlist.org/t/how-does-pmt-creates-pixels/2838)
**Author:** [@deepak\_jangra](https://forum.microlist.org/u/deepak_jangra)\ **Replies:** 5\ **Last updated:** [February 28, 2025, 4:28pm UTC](https://forum.microlist.org/t/how-does-pmt-creates-pixels/2838 "2025-02-28T16:28:17Z")
How actually the pixels are created by PMT detectors in confocal microscopes? I understand till signal amplification by dynodes inside PMT and voltage generation at Anode, but how this voltage is transformed into Pixels… --- ## [How am I obtaining higher energy photons from a fluorescein solution than the ones I'm exciting it with?](https://forum.microlist.org/t/how-am-i-obtaining-higher-energy-photons-from-a-fluorescein-solution-than-the-ones-im-exciting-it-with/3010)
**Author:** [@JelleP](https://forum.microlist.org/u/JelleP)\ **Replies:** 3\ **Last updated:** [February 28, 2025, 4:24pm UTC](https://forum.microlist.org/t/how-am-i-obtaining-higher-energy-photons-from-a-fluorescein-solution-than-the-ones-im-exciting-it-with/3010 "2025-02-28T16:24:27Z")
Hi all, I have a theory question that is probably obvious to some of you! I’m confocal scanning a FITC solution as follows: Imgur: The magic of the Internet with excitation at 520 nm and emission around 500 nm. I’m usi… --- ## [405-nm excitation conjugated phalloidin](https://forum.microlist.org/t/405-nm-excitation-conjugated-phalloidin/1788)
**Author:** [@cleterrier](https://forum.microlist.org/u/cleterrier)\ **Replies:** 1\ **Last updated:** [February 20, 2025, 11:10am UTC](https://forum.microlist.org/t/405-nm-excitation-conjugated-phalloidin/1788 "2025-02-20T11:10:50Z")
SIM/confocal folks, what’s the latest on usable fluorescent phalloidin with a 405 nm laser line? Our preferred option (DyLight 405) is not available. AF405+? Atto something? CF405M? iFluor 405? I don’t think Brilliant Vi… --- ## [Guide to Microscopy about Cellular Ploidy Variation](https://forum.microlist.org/t/guide-to-microscopy-about-cellular-ploidy-variation/2301)
**Author:** [@Matheus\_C\_Casotti](https://forum.microlist.org/u/Matheus_C_Casotti)\ **Replies:** 2\ **Last updated:** [February 13, 2025, 8:54pm UTC](https://forum.microlist.org/t/guide-to-microscopy-about-cellular-ploidy-variation/2301 "2025-02-13T20:54:47Z")
Good morning My name is Matheus, I’m starting out in the area of image analysis and I would like to develop an analysis and identification of polyploid cells in histological slides stained in HE and also with fluorescen… --- ## [Laser alignment and resolution](https://forum.microlist.org/t/laser-alignment-and-resolution/2974)
**Author:** [@szh141](https://forum.microlist.org/u/szh141)\ **Replies:** 2\ **Last updated:** [January 27, 2025, 8:21pm UTC](https://forum.microlist.org/t/laser-alignment-and-resolution/2974 "2025-01-27T20:21:11Z")
Hello. I have a question about the relationship between laser alignment and the resulted image resolution. I imaged DAPI channel of a fixed brain section at 20x/0.8 on a point scanning confocal. It’s 1 AU on the green c… --- ## [WITec Alpha300R from Oxford Instruments](https://forum.microlist.org/t/witec-alpha300r-from-oxford-instruments/2943)
**Author:** [@Wojciech](https://forum.microlist.org/u/Wojciech)\ **Replies:** 2\ **Last updated:** [January 5, 2025, 11:45am UTC](https://forum.microlist.org/t/witec-alpha300r-from-oxford-instruments/2943 "2025-01-05T11:45:00Z")
Dear forum Members, I came across some public tenders that require updates or expansions related to new laser sources to existing confocal microscopes. For example the purchase of two Raman lasers at 633 nm and 785 nm … --- ## [Airyscan imaging issues](https://forum.microlist.org/t/airyscan-imaging-issues/2940)
**Author:** [@bufobufo](https://forum.microlist.org/u/bufobufo)\ **Replies:** 3\ **Last updated:** [December 31, 2024, 4:46am UTC](https://forum.microlist.org/t/airyscan-imaging-issues/2940 "2024-12-31T04:46:40Z")
Hi, I am a PhD student, and my project heavily depends on imaging. I’ve been using LSM880 for my confocal images; however, recently, my PI suggested I start using airyscan for some of my images. I am trying to visualize… --- ## [Crest X-light V3 spinning disk confocal reviews](https://forum.microlist.org/t/crest-x-light-v3-spinning-disk-confocal-reviews/565)
**Author:** [@Andrew\_Seeber](https://forum.microlist.org/u/Andrew_Seeber)\ **Replies:** 27\ **Last updated:** [December 20, 2024, 7:03am UTC](https://forum.microlist.org/t/crest-x-light-v3-spinning-disk-confocal-reviews/565 "2024-12-20T07:03:05Z")
Hi all, I’m wondering if anyone has used or owns a X-light V3 spinning disk? They are very competitively priced against the Andor Dragonfly and Yokogawa W1 but I’m a little skeptical. I’d love to hear experiences and op… --- ## [Olympus ix73 reuse/rebuild](https://forum.microlist.org/t/olympus-ix73-reuse-rebuild/2373)
**Author:** [@Daryll\_Dalayoan](https://forum.microlist.org/u/Daryll_Dalayoan)\ **Replies:** 2\ **Last updated:** [November 26, 2024, 2:46am UTC](https://forum.microlist.org/t/olympus-ix73-reuse-rebuild/2373 "2024-11-26T02:46:40Z")
Hi guys was wondering if you can give me direction on how should I go about reusing this ix73 microscope. I got tasked in my lab to reuse/ rebuild this microscope. We plan to use it for epi fluorescence application: PL a… [Next page](https://forum.microlist.org/tag/confocal/25.md?match_all_tags=true&page=1&tags%5B%5D=confocal)